Endogenous cannabinoids contribute to remote ischemic preconditioning via cannabinoid CB2 receptors in the rat heart

Amir R. Hajrasouliha, Sina Tavakoli, Mehdi Ghasemi, Pejman Jabehdar-Maralani, Hamed Sadeghipour, Farzad Ebrahimi, Ahmad Reza Dehpour

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

In addition to well-known neurobehavioral effects, endogenous cannabinoids exert diverse cardiovascular actions. Recently, they have been suggested to protect the myocardium against ischemia/reperfusion injury. The aim of this study is to examine the contribution of endogenous cannabinoids to cardioprotection afforded by remote ischemic preconditioning. Three groups of remote preconditioned (15 min of mesenteric artery occlusion followed by 15 min of reperfusion) and three groups of sham-operated rats were included in the study. Animals were pretreated intravenously by vehicle, cannabinoid CB1 (AM251, 1 mg/kg) or CB2 (AM630, 1 mg/kg) receptor antagonist 15 min prior to remote preconditioning or sham operation. Myocardial injury was induced by 30 min of coronary artery occlusion followed by 2 h of reperfusion. The resultant arterial hypotension, ventricular arrhythmias, and infarct size were compared among the groups. Remote preconditioning exerted potent cardioprotection manifested as significant reductions in infarct size (P < 0.001) as well as number and duration of arrhythmias (P < 0.01, 0.01 and 0.05 for premature ventricular contractions, ventricular tachycardias and fibrillations; respectively). The cannabinoid CB1 receptor antagonist pretreatment had no significant effect on ischemia-induced hypotension, arrhythmias or infarct size. On the other hand, the cannabinoid CB2 receptor antagonist pretreatment abolished the protective effects of remote preconditioning on infarct size (P < 0.01) and arrhythmias (P < 0.01), without any significant effect on ischemia-induced hypotension. The results of this study suggest that endogenous cannabinoids, through acting on cannabinoid CB2 receptors, are involved in the cardioprotective phenomenon of remote ischemic preconditioning, induced by mesenteric artery occlusion and reperfusion.

Original languageEnglish (US)
Pages (from-to)246-252
Number of pages7
JournalEuropean Journal of Pharmacology
Volume579
Issue number1-3
DOIs
StatePublished - Jan 28 2008
Externally publishedYes

Fingerprint

Ischemic Preconditioning
Cannabinoids
Cardiac Arrhythmias
Controlled Hypotension
Cannabinoid Receptor Antagonists
Reperfusion
Mesenteric Arteries
Ischemia
Cannabinoid Receptor CB2
Ventricular Premature Complexes
Coronary Occlusion
Ventricular Fibrillation
Ventricular Tachycardia
Reperfusion Injury
Hypotension
Coronary Vessels
Myocardium
rat Cnr2 protein
Wounds and Injuries

Keywords

  • Arrhythmia
  • Endogenous cannabinoid
  • Ischemia/reperfusion
  • Necrosis
  • Remote preconditioning

ASJC Scopus subject areas

  • Pharmacology

Cite this

Endogenous cannabinoids contribute to remote ischemic preconditioning via cannabinoid CB2 receptors in the rat heart. / Hajrasouliha, Amir R.; Tavakoli, Sina; Ghasemi, Mehdi; Jabehdar-Maralani, Pejman; Sadeghipour, Hamed; Ebrahimi, Farzad; Dehpour, Ahmad Reza.

In: European Journal of Pharmacology, Vol. 579, No. 1-3, 28.01.2008, p. 246-252.

Research output: Contribution to journalArticle

Hajrasouliha, Amir R. ; Tavakoli, Sina ; Ghasemi, Mehdi ; Jabehdar-Maralani, Pejman ; Sadeghipour, Hamed ; Ebrahimi, Farzad ; Dehpour, Ahmad Reza. / Endogenous cannabinoids contribute to remote ischemic preconditioning via cannabinoid CB2 receptors in the rat heart. In: European Journal of Pharmacology. 2008 ; Vol. 579, No. 1-3. pp. 246-252.
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