Endothelial abnormalities in adolescents with type 1 diabetes

A biomarker for vascular sequelae?

Linda DiMeglio, Aneesh Tosh, Chandan Saha, Myka Estes, Julie Mund, Laura E. Mead, Izlin Lien, David Ingram, Laura Haneline

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Objective: To evaluate whether counts of circulating colony forming unit-endothelial cells (CFU-ECs), cells co-expressing CD34, CD133, and CD31 (CD34+CD133+CD31+), and CD34+CD45- cells are altered in adolescents with type 1 diabetes and if the changes in counts correlate with endothelial dysfunction. Study design: Adolescents with diabetes (ages 18 to 22 years) and race- and sex-matched control subjects were studied. We assessed circulating CFU-ECs, using colony assays, and CD34+CD133+CD31+ and CD34+CD45- cells, using poly-chromatic flow cytometry. CFU-ECs and CD34+CD133+CD31+ are hematopoietic-derived progenitors that inversely correlate with cardiovascular risk in adults. CD34+CD45- cells are enriched for endothelial cells with robust vasculogenic potential. Vascular reactivity was tested by laser Doppler iontophoresis. Results: Subjects with diabetes had lower CD34+CD133+CD31+ cells, a trend toward reduced CFU-ECs, and increased CD34+CD45- cells compared with control subjects. Endothelium-dependent vasodilation was impaired in subjects with diabetes, which correlated with reductions in circulating CD34+CD133+CD31+ cells. Conclusions: Long-term sequelae of type 1 diabetes include vasculopathies. Endothelial progenitor cells promote vascular health by facilitating endothelial integrity and function. Lower CD34+CD133+CD31+ cells may be a harbinger of future macrovascular disease risk. Higher circulating CD34+CD45- cells may reflect ongoing endothelial damage. These cells are potential biomarkers to guide therapeutic interventions to enhance endothelial function and to prevent progression to overt vascular disease.

Original languageEnglish
Pages (from-to)540-546
Number of pages7
JournalJournal of Pediatrics
Volume157
Issue number4
DOIs
StatePublished - Oct 2010

Fingerprint

Type 1 Diabetes Mellitus
Blood Vessels
Biomarkers
Endothelial Cells
Stem Cells
Iontophoresis
Vascular Diseases
Vasodilation
Endothelium
Flow Cytometry
Lasers
Color
Health

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Medicine(all)

Cite this

Endothelial abnormalities in adolescents with type 1 diabetes : A biomarker for vascular sequelae? / DiMeglio, Linda; Tosh, Aneesh; Saha, Chandan; Estes, Myka; Mund, Julie; Mead, Laura E.; Lien, Izlin; Ingram, David; Haneline, Laura.

In: Journal of Pediatrics, Vol. 157, No. 4, 10.2010, p. 540-546.

Research output: Contribution to journalArticle

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