Endothelial colony-forming cells ameliorate endothelial dysfunction via secreted factors following ischemia-reperfusion injury

Jason A. Collett, Purvi Mehrotra, Allison Crone, W. Shelley Christopher, Mervin Yoder, David Basile

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Damage to endothelial cells contributes to acute kidney injury (AKI) by leading to impaired perfusion. Endothelial colony-forming cells (ECFC) are endothelial precursor cells with high proliferative capacity, pro-angiogenic activity, and in vivo vessel forming potential. We hypothesized that ECFC may ameliorate the degree of AKI and/or promote repair of the renal vasculature following ischemia-reperfusion (I/R). Rat pulmonary microvascular endothelial cells (PMVEC) with high proliferative potential were compared with pulmonary artery endothelial cells (PAEC) with low proliferative potential in rats subjected to renal I/R. PMVEC administration reduced renal injury and hastened recovery as indicated by serum creatinine and tubular injury scores, while PAEC did not. Vehicle-treated control animals showed consistent reductions in renal medullary blood flow (MBF) within 2 h of reperfusion, while PMVEC protected against loss in MBF as measured by laser Doppler. Interestingly, PMVEC mediated protection occurred in the absence of homing to the kidney. Conditioned medium (CM) from human cultured cord blood ECFC also conveyed beneficial effects against I/R injury and loss of MBF. Moreover, ECFC-CM significantly reduced the expression of ICAM-1 and decreased the number of differentiated lymphocytes typically recruited into the kidney following renal ischemia. Taken together, these data suggest that ECFC secrete factors that preserve renal function post ischemia, in part, by preserving microvascular function.

Original languageEnglish (US)
Pages (from-to)F897-F907
JournalAmerican Journal of Physiology - Renal Physiology
Volume312
Issue number5
DOIs
StatePublished - May 15 2017

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Reperfusion Injury
Endothelial Cells
Kidney
Lung
Ischemia
Conditioned Culture Medium
Acute Kidney Injury
Pulmonary Artery
Reperfusion
Cytoprotection
Renal Circulation
Wounds and Injuries
Lymphocyte Count
Intercellular Adhesion Molecule-1
Fetal Blood
Creatinine
Lasers
Perfusion
Serum

Keywords

  • Angiogenesis
  • Endothelial progenitor
  • Hemodynamics
  • Regeneration

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Endothelial colony-forming cells ameliorate endothelial dysfunction via secreted factors following ischemia-reperfusion injury. / Collett, Jason A.; Mehrotra, Purvi; Crone, Allison; Christopher, W. Shelley; Yoder, Mervin; Basile, David.

In: American Journal of Physiology - Renal Physiology, Vol. 312, No. 5, 15.05.2017, p. F897-F907.

Research output: Contribution to journalArticle

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