Endothelial colony-forming cells: Biological and functional abnormalities in patients with recurrent, unprovoked venous thromboembolic disease

Jose Antonio Alvarado-Moreno, Rubicel Hernandez-Lopez, Antonieta Chavez-Gonzalez, Mervin Yoder, Rosalva Rangel-Corona, Irma Isordia-Salas, Jesus Hernandez-Juarez, Arturo Cerbulo-Vazquez, Marco Antonio Gonzalez-Jimenez, Abraham Majluf-Cruz

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Introduction Endothelial cells (ECs) are an important component of the blood coagulation system because it maintains blood fluid. Because in patients with venous thromboembolic disease (VTD) a thrombophilic condition is not found sometimes, we investigated if endothelial colony-forming cells (ECFCs) from these patients have biological and functional abnormalities. Patients and methods Human mononuclear cells (MNCs) were obtained from peripheral blood from patients with VTD and controls to obtain ECFCs. These cells were assayed for their immunophenotype and electron microscopy characteristics and their ability to form capillary-like structures and to produce pro-inflammatory and pro-angiogenic cytokines and reactive oxygen species (ROS). Results ECFCs appeared at 7 and 21 days of culture in VTD patients and controls, respectively. ECFCs increased 8-fold in patients and emerged 1 week earlier. No differences in the size of the colonies of ECFCs were found. Numbers and time of appearance of ECFCs was different between groups. ECFC-derived ECs (ECFC-ECs) of both groups expressed CD31, CD34, CD146, and CD-309 but none expressed CD45, CD14, or CD90. Interest CD34 was highly expressed in ECFC-ECs from patients. In both groups, ECFC-ECs showed similar capacity to form capillary-like structures but ECFC-ECs from patients had significant abnormalities in the mitochondrial membrane. We found a significant increase in ROS production in ECFC-ECs from patients. There were significant differences in cytokine profiles between VTD patients and controls. Conclusions We found a dysfunctional state in ECFC from VTD patients resembling some characteristics of dysfunctional ECs. These findings may help to understand some pathophysiological aspects of VTD.

Original languageEnglish (US)
Pages (from-to)157-168
Number of pages12
JournalThrombosis Research
Volume137
DOIs
StatePublished - Jan 1 2016

Fingerprint

Endothelial Cells
Reactive Oxygen Species
Cytokines
Mitochondrial Membranes
Blood Coagulation
Cellular Structures
Electron Microscopy

Keywords

  • Deep vein thrombosis
  • Endothelial cells
  • Endothelial colony-forming cells
  • Thrombosis
  • Venous thromboembolic disease

ASJC Scopus subject areas

  • Hematology

Cite this

Alvarado-Moreno, J. A., Hernandez-Lopez, R., Chavez-Gonzalez, A., Yoder, M., Rangel-Corona, R., Isordia-Salas, I., ... Majluf-Cruz, A. (2016). Endothelial colony-forming cells: Biological and functional abnormalities in patients with recurrent, unprovoked venous thromboembolic disease. Thrombosis Research, 137, 157-168. https://doi.org/10.1016/j.thromres.2015.11.005

Endothelial colony-forming cells : Biological and functional abnormalities in patients with recurrent, unprovoked venous thromboembolic disease. / Alvarado-Moreno, Jose Antonio; Hernandez-Lopez, Rubicel; Chavez-Gonzalez, Antonieta; Yoder, Mervin; Rangel-Corona, Rosalva; Isordia-Salas, Irma; Hernandez-Juarez, Jesus; Cerbulo-Vazquez, Arturo; Gonzalez-Jimenez, Marco Antonio; Majluf-Cruz, Abraham.

In: Thrombosis Research, Vol. 137, 01.01.2016, p. 157-168.

Research output: Contribution to journalArticle

Alvarado-Moreno, JA, Hernandez-Lopez, R, Chavez-Gonzalez, A, Yoder, M, Rangel-Corona, R, Isordia-Salas, I, Hernandez-Juarez, J, Cerbulo-Vazquez, A, Gonzalez-Jimenez, MA & Majluf-Cruz, A 2016, 'Endothelial colony-forming cells: Biological and functional abnormalities in patients with recurrent, unprovoked venous thromboembolic disease', Thrombosis Research, vol. 137, pp. 157-168. https://doi.org/10.1016/j.thromres.2015.11.005
Alvarado-Moreno, Jose Antonio ; Hernandez-Lopez, Rubicel ; Chavez-Gonzalez, Antonieta ; Yoder, Mervin ; Rangel-Corona, Rosalva ; Isordia-Salas, Irma ; Hernandez-Juarez, Jesus ; Cerbulo-Vazquez, Arturo ; Gonzalez-Jimenez, Marco Antonio ; Majluf-Cruz, Abraham. / Endothelial colony-forming cells : Biological and functional abnormalities in patients with recurrent, unprovoked venous thromboembolic disease. In: Thrombosis Research. 2016 ; Vol. 137. pp. 157-168.
@article{27e4cc1fe2a24281abaac6deb31cb6d2,
title = "Endothelial colony-forming cells: Biological and functional abnormalities in patients with recurrent, unprovoked venous thromboembolic disease",
abstract = "Introduction Endothelial cells (ECs) are an important component of the blood coagulation system because it maintains blood fluid. Because in patients with venous thromboembolic disease (VTD) a thrombophilic condition is not found sometimes, we investigated if endothelial colony-forming cells (ECFCs) from these patients have biological and functional abnormalities. Patients and methods Human mononuclear cells (MNCs) were obtained from peripheral blood from patients with VTD and controls to obtain ECFCs. These cells were assayed for their immunophenotype and electron microscopy characteristics and their ability to form capillary-like structures and to produce pro-inflammatory and pro-angiogenic cytokines and reactive oxygen species (ROS). Results ECFCs appeared at 7 and 21 days of culture in VTD patients and controls, respectively. ECFCs increased 8-fold in patients and emerged 1 week earlier. No differences in the size of the colonies of ECFCs were found. Numbers and time of appearance of ECFCs was different between groups. ECFC-derived ECs (ECFC-ECs) of both groups expressed CD31, CD34, CD146, and CD-309 but none expressed CD45, CD14, or CD90. Interest CD34 was highly expressed in ECFC-ECs from patients. In both groups, ECFC-ECs showed similar capacity to form capillary-like structures but ECFC-ECs from patients had significant abnormalities in the mitochondrial membrane. We found a significant increase in ROS production in ECFC-ECs from patients. There were significant differences in cytokine profiles between VTD patients and controls. Conclusions We found a dysfunctional state in ECFC from VTD patients resembling some characteristics of dysfunctional ECs. These findings may help to understand some pathophysiological aspects of VTD.",
keywords = "Deep vein thrombosis, Endothelial cells, Endothelial colony-forming cells, Thrombosis, Venous thromboembolic disease",
author = "Alvarado-Moreno, {Jose Antonio} and Rubicel Hernandez-Lopez and Antonieta Chavez-Gonzalez and Mervin Yoder and Rosalva Rangel-Corona and Irma Isordia-Salas and Jesus Hernandez-Juarez and Arturo Cerbulo-Vazquez and Gonzalez-Jimenez, {Marco Antonio} and Abraham Majluf-Cruz",
year = "2016",
month = "1",
day = "1",
doi = "10.1016/j.thromres.2015.11.005",
language = "English (US)",
volume = "137",
pages = "157--168",
journal = "Thrombosis Research",
issn = "0049-3848",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Endothelial colony-forming cells

T2 - Biological and functional abnormalities in patients with recurrent, unprovoked venous thromboembolic disease

AU - Alvarado-Moreno, Jose Antonio

AU - Hernandez-Lopez, Rubicel

AU - Chavez-Gonzalez, Antonieta

AU - Yoder, Mervin

AU - Rangel-Corona, Rosalva

AU - Isordia-Salas, Irma

AU - Hernandez-Juarez, Jesus

AU - Cerbulo-Vazquez, Arturo

AU - Gonzalez-Jimenez, Marco Antonio

AU - Majluf-Cruz, Abraham

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Introduction Endothelial cells (ECs) are an important component of the blood coagulation system because it maintains blood fluid. Because in patients with venous thromboembolic disease (VTD) a thrombophilic condition is not found sometimes, we investigated if endothelial colony-forming cells (ECFCs) from these patients have biological and functional abnormalities. Patients and methods Human mononuclear cells (MNCs) were obtained from peripheral blood from patients with VTD and controls to obtain ECFCs. These cells were assayed for their immunophenotype and electron microscopy characteristics and their ability to form capillary-like structures and to produce pro-inflammatory and pro-angiogenic cytokines and reactive oxygen species (ROS). Results ECFCs appeared at 7 and 21 days of culture in VTD patients and controls, respectively. ECFCs increased 8-fold in patients and emerged 1 week earlier. No differences in the size of the colonies of ECFCs were found. Numbers and time of appearance of ECFCs was different between groups. ECFC-derived ECs (ECFC-ECs) of both groups expressed CD31, CD34, CD146, and CD-309 but none expressed CD45, CD14, or CD90. Interest CD34 was highly expressed in ECFC-ECs from patients. In both groups, ECFC-ECs showed similar capacity to form capillary-like structures but ECFC-ECs from patients had significant abnormalities in the mitochondrial membrane. We found a significant increase in ROS production in ECFC-ECs from patients. There were significant differences in cytokine profiles between VTD patients and controls. Conclusions We found a dysfunctional state in ECFC from VTD patients resembling some characteristics of dysfunctional ECs. These findings may help to understand some pathophysiological aspects of VTD.

AB - Introduction Endothelial cells (ECs) are an important component of the blood coagulation system because it maintains blood fluid. Because in patients with venous thromboembolic disease (VTD) a thrombophilic condition is not found sometimes, we investigated if endothelial colony-forming cells (ECFCs) from these patients have biological and functional abnormalities. Patients and methods Human mononuclear cells (MNCs) were obtained from peripheral blood from patients with VTD and controls to obtain ECFCs. These cells were assayed for their immunophenotype and electron microscopy characteristics and their ability to form capillary-like structures and to produce pro-inflammatory and pro-angiogenic cytokines and reactive oxygen species (ROS). Results ECFCs appeared at 7 and 21 days of culture in VTD patients and controls, respectively. ECFCs increased 8-fold in patients and emerged 1 week earlier. No differences in the size of the colonies of ECFCs were found. Numbers and time of appearance of ECFCs was different between groups. ECFC-derived ECs (ECFC-ECs) of both groups expressed CD31, CD34, CD146, and CD-309 but none expressed CD45, CD14, or CD90. Interest CD34 was highly expressed in ECFC-ECs from patients. In both groups, ECFC-ECs showed similar capacity to form capillary-like structures but ECFC-ECs from patients had significant abnormalities in the mitochondrial membrane. We found a significant increase in ROS production in ECFC-ECs from patients. There were significant differences in cytokine profiles between VTD patients and controls. Conclusions We found a dysfunctional state in ECFC from VTD patients resembling some characteristics of dysfunctional ECs. These findings may help to understand some pathophysiological aspects of VTD.

KW - Deep vein thrombosis

KW - Endothelial cells

KW - Endothelial colony-forming cells

KW - Thrombosis

KW - Venous thromboembolic disease

UR - http://www.scopus.com/inward/record.url?scp=84952637752&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84952637752&partnerID=8YFLogxK

U2 - 10.1016/j.thromres.2015.11.005

DO - 10.1016/j.thromres.2015.11.005

M3 - Article

C2 - 26597044

AN - SCOPUS:84952637752

VL - 137

SP - 157

EP - 168

JO - Thrombosis Research

JF - Thrombosis Research

SN - 0049-3848

ER -