Endothelial colony-forming cells from preterm infants are increased and more susceptible to hyperoxia

Christopher D. Baker, Sharon L. Ryan, David A. Ingram, Gregory J. Seedorf, Steven H. Abman, Vivek Balasubramaniam

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Rationale: Preterm birth and hyperoxic exposure increase the risk for bronchopulmonary dysplasia (BPD), a chronic lung disease characterized by impaired vascular and alveolar growth. Endothelial progenitor cells, such as self-renewing highly proliferative endothelial colony-forming cells (ECFCs), may participate in vascular repair. The effect of hyperoxia on ECFC growth is unknown. Objectives: We hypothesize that umbilical cord blood (CB) from premature infants contains more ECFCs with greater growth potential than term CB. However, preterm ECFCs may be more susceptible to hyperoxia. Methods: ECFC colonies were quantified by established methods and characterized by immunohistochemistry and flow cytometry. Growth kinetics were assessed in room air and hyperoxia (FIO2 = 0.4). Measurements and Main Results: Preterm CB (28-35 wk gestation) yielded significantly more ECFC colonies than term CB. Importantly, we found that CD45-/CD34+/CD133 +/VEGFR-2+ cell number did not correlate with ECFC colony count. Preterm ECFCs demonstrated increased growth compared with term ECFCs. Hyperoxia impaired growth of preterm but not term ECFCs. Treatment with superoxide dismutase and catalase enhanced preterm ECFC growth during hyperoxia. Conclusions: Preterm ECFCs appear in increased numbers and proliferate more rapidly but have an increased susceptibility to hyperoxia compared with term ECFCs. Antioxidants protect preterm ECFCs from hyperoxia.

Original languageEnglish (US)
Pages (from-to)454-461
Number of pages8
JournalAmerican journal of respiratory and critical care medicine
Volume180
Issue number5
DOIs
StatePublished - Sep 1 2009

Fingerprint

Hyperoxia
Premature Infants
Fetal Blood
Growth
Blood Vessels
Cell Count
Vascular Endothelial Growth Factor Receptor-2
Bronchopulmonary Dysplasia
Premature Birth
Catalase
Lung Diseases
Superoxide Dismutase
Flow Cytometry
Chronic Disease

Keywords

  • Antioxidants
  • Bronchopulmonary dysplasia
  • Endothelial progenitor cells
  • Reactive oxygen species
  • Umbilical cord blood

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Endothelial colony-forming cells from preterm infants are increased and more susceptible to hyperoxia. / Baker, Christopher D.; Ryan, Sharon L.; Ingram, David A.; Seedorf, Gregory J.; Abman, Steven H.; Balasubramaniam, Vivek.

In: American journal of respiratory and critical care medicine, Vol. 180, No. 5, 01.09.2009, p. 454-461.

Research output: Contribution to journalArticle

Baker, Christopher D. ; Ryan, Sharon L. ; Ingram, David A. ; Seedorf, Gregory J. ; Abman, Steven H. ; Balasubramaniam, Vivek. / Endothelial colony-forming cells from preterm infants are increased and more susceptible to hyperoxia. In: American journal of respiratory and critical care medicine. 2009 ; Vol. 180, No. 5. pp. 454-461.
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