Endothelial monocyte-activating polypeptide II. A novel tumor-derived polypeptide that activates host-response mechanisms

J. Kao, J. Ryan, G. Brett, J. Chen, H. Shen, Y. G. Fan, G. Godman, P. C. Familletti, F. Wang, Y. C.E. Pan, D. Stern, M. Clauss

Research output: Contribution to journalArticle

161 Scopus citations

Abstract

An important means by which tumor cells influence the vasculature is through the production of soluble mediators altering vascular properties. A ≃22-kDa polypeptide was purified to homogeneity from conditioned medium of murine methylcholanthrene A (meth A) fibrosarcoma cells by ion-exchange chromatography and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), based on its ability to induce tissue factor procoagulant activity in endothelial cells (ECs). The final product migrated as a broad band on reduced and nonreduced SDS-PAGE and had a unique amino- terminal sequence. This meth A-derived polypeptide modulated EC coagulant properties through the induction of tissue factor, induced monocyte migration and tissue factor expression, and was also chemotactic for granulocytes. Injection of the polypeptide into mouse footpads resulted in an inflammatory response with tissue swelling and polymorphonuclear leukocyte infiltration. The ability of this mediator to activate ECs and monocytes has led us to name it EMAP II (endothelial monocyte-activating polypeptide). EMAP II is distinct from a previously described ≃40-kDa meth A-derived polypeptide termed EMAP I. Through its potential to activate host effector mechanisms, EMAP II could contribute to the biology of immunogenic tumors, such as the meth A fibrosarcoma.

Original languageEnglish (US)
Pages (from-to)20239-20247
Number of pages9
JournalJournal of Biological Chemistry
Volume267
Issue number28
StatePublished - Jan 1 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Kao, J., Ryan, J., Brett, G., Chen, J., Shen, H., Fan, Y. G., Godman, G., Familletti, P. C., Wang, F., Pan, Y. C. E., Stern, D., & Clauss, M. (1992). Endothelial monocyte-activating polypeptide II. A novel tumor-derived polypeptide that activates host-response mechanisms. Journal of Biological Chemistry, 267(28), 20239-20247.