Endothelial seeding of Dacron and polytetrafluoroethylene grafts: The cellular events of healing

Malcolm Herring, Sally Baughman, John Glover, Kenneth Kesler, Jerry Jesseph, Jessica Campbell, Russell Dilley, Andrew Evan, Austin Gardner

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

To detect cellular differences in the healing of polytetrafluoroethylene (e-PTFE) and Dacron grafts up to 7 months after implantation, we studied 108 aortic graft interpositions in dogs. Each prosthesis was alternately prepared by endothelial seeding or by an unseeded control method. The grafts were perfusion fixed and studied with light, scanning, and transmission electron microscopy at intervals from before to 221 days after implantation. Seeding resulted in the development of an extensive endothelial flow surface in two out of three of the e-PTFE and none out of four of the Dacron grafts by 10 days after implantation (p = 0.053). After 30 days a microfibrillar subendothelial matrix ranging from 5 to 11 μ formed in all but three grafts with endothelial coverage. The inner capsule of mature Dacron grafts was significantly thicker (169 ± 143 μ) than in e-PTFE grafts (22 ± 32 μ; p = 0.002). Seeded and unseeded Dacron grafts had predominantly fibroblasts in the outer capsule of the graft by 10 days. Surface endothelium, vasa vasorum, fibroblasts, and myointimal cells appeared in the inner capsule between 10 and 30 days after implantation. In Dacron grafts, fibroblasts and myointimal cells predominated in the inner capsule at 30 days, with smooth muscle cells not being definitely identifiable until after 150 days. Neither fibroblasts nor myointimal cells were common (present but sparce in one of four e-PTFE grafts) at 30 days, and transmural vasa vasorum were never seen. The seeded endothelial cells migrated rapidly from the sites of initial adhesion near the e-PTFE onto the flow surface. Only one of four of the unseeded e-PTFE grafts had surface endothelium after 30 days, and only moderate coverage developed during 180 days. We conclude that endothelial healing is more rapid m seeded e-PTFE grafts than in seeded Dacron grafts and occurs by a different mechanism.

Original languageEnglish
Pages (from-to)745-755
Number of pages11
JournalSurgery
Volume96
Issue number4
StatePublished - 1984

Fingerprint

Polyethylene Terephthalates
Polytetrafluoroethylene
Transplants
Capsules
Fibroblasts
Vasa Vasorum
Endothelium
Scanning Transmission Electron Microscopy
Prostheses and Implants
Smooth Muscle Myocytes

ASJC Scopus subject areas

  • Surgery

Cite this

Herring, M., Baughman, S., Glover, J., Kesler, K., Jesseph, J., Campbell, J., ... Gardner, A. (1984). Endothelial seeding of Dacron and polytetrafluoroethylene grafts: The cellular events of healing. Surgery, 96(4), 745-755.

Endothelial seeding of Dacron and polytetrafluoroethylene grafts : The cellular events of healing. / Herring, Malcolm; Baughman, Sally; Glover, John; Kesler, Kenneth; Jesseph, Jerry; Campbell, Jessica; Dilley, Russell; Evan, Andrew; Gardner, Austin.

In: Surgery, Vol. 96, No. 4, 1984, p. 745-755.

Research output: Contribution to journalArticle

Herring, M, Baughman, S, Glover, J, Kesler, K, Jesseph, J, Campbell, J, Dilley, R, Evan, A & Gardner, A 1984, 'Endothelial seeding of Dacron and polytetrafluoroethylene grafts: The cellular events of healing', Surgery, vol. 96, no. 4, pp. 745-755.
Herring M, Baughman S, Glover J, Kesler K, Jesseph J, Campbell J et al. Endothelial seeding of Dacron and polytetrafluoroethylene grafts: The cellular events of healing. Surgery. 1984;96(4):745-755.
Herring, Malcolm ; Baughman, Sally ; Glover, John ; Kesler, Kenneth ; Jesseph, Jerry ; Campbell, Jessica ; Dilley, Russell ; Evan, Andrew ; Gardner, Austin. / Endothelial seeding of Dacron and polytetrafluoroethylene grafts : The cellular events of healing. In: Surgery. 1984 ; Vol. 96, No. 4. pp. 745-755.
@article{4ed7799dab42464f8593be0eb58fa84e,
title = "Endothelial seeding of Dacron and polytetrafluoroethylene grafts: The cellular events of healing",
abstract = "To detect cellular differences in the healing of polytetrafluoroethylene (e-PTFE) and Dacron grafts up to 7 months after implantation, we studied 108 aortic graft interpositions in dogs. Each prosthesis was alternately prepared by endothelial seeding or by an unseeded control method. The grafts were perfusion fixed and studied with light, scanning, and transmission electron microscopy at intervals from before to 221 days after implantation. Seeding resulted in the development of an extensive endothelial flow surface in two out of three of the e-PTFE and none out of four of the Dacron grafts by 10 days after implantation (p = 0.053). After 30 days a microfibrillar subendothelial matrix ranging from 5 to 11 μ formed in all but three grafts with endothelial coverage. The inner capsule of mature Dacron grafts was significantly thicker (169 ± 143 μ) than in e-PTFE grafts (22 ± 32 μ; p = 0.002). Seeded and unseeded Dacron grafts had predominantly fibroblasts in the outer capsule of the graft by 10 days. Surface endothelium, vasa vasorum, fibroblasts, and myointimal cells appeared in the inner capsule between 10 and 30 days after implantation. In Dacron grafts, fibroblasts and myointimal cells predominated in the inner capsule at 30 days, with smooth muscle cells not being definitely identifiable until after 150 days. Neither fibroblasts nor myointimal cells were common (present but sparce in one of four e-PTFE grafts) at 30 days, and transmural vasa vasorum were never seen. The seeded endothelial cells migrated rapidly from the sites of initial adhesion near the e-PTFE onto the flow surface. Only one of four of the unseeded e-PTFE grafts had surface endothelium after 30 days, and only moderate coverage developed during 180 days. We conclude that endothelial healing is more rapid m seeded e-PTFE grafts than in seeded Dacron grafts and occurs by a different mechanism.",
author = "Malcolm Herring and Sally Baughman and John Glover and Kenneth Kesler and Jerry Jesseph and Jessica Campbell and Russell Dilley and Andrew Evan and Austin Gardner",
year = "1984",
language = "English",
volume = "96",
pages = "745--755",
journal = "Surgery",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "4",

}

TY - JOUR

T1 - Endothelial seeding of Dacron and polytetrafluoroethylene grafts

T2 - The cellular events of healing

AU - Herring, Malcolm

AU - Baughman, Sally

AU - Glover, John

AU - Kesler, Kenneth

AU - Jesseph, Jerry

AU - Campbell, Jessica

AU - Dilley, Russell

AU - Evan, Andrew

AU - Gardner, Austin

PY - 1984

Y1 - 1984

N2 - To detect cellular differences in the healing of polytetrafluoroethylene (e-PTFE) and Dacron grafts up to 7 months after implantation, we studied 108 aortic graft interpositions in dogs. Each prosthesis was alternately prepared by endothelial seeding or by an unseeded control method. The grafts were perfusion fixed and studied with light, scanning, and transmission electron microscopy at intervals from before to 221 days after implantation. Seeding resulted in the development of an extensive endothelial flow surface in two out of three of the e-PTFE and none out of four of the Dacron grafts by 10 days after implantation (p = 0.053). After 30 days a microfibrillar subendothelial matrix ranging from 5 to 11 μ formed in all but three grafts with endothelial coverage. The inner capsule of mature Dacron grafts was significantly thicker (169 ± 143 μ) than in e-PTFE grafts (22 ± 32 μ; p = 0.002). Seeded and unseeded Dacron grafts had predominantly fibroblasts in the outer capsule of the graft by 10 days. Surface endothelium, vasa vasorum, fibroblasts, and myointimal cells appeared in the inner capsule between 10 and 30 days after implantation. In Dacron grafts, fibroblasts and myointimal cells predominated in the inner capsule at 30 days, with smooth muscle cells not being definitely identifiable until after 150 days. Neither fibroblasts nor myointimal cells were common (present but sparce in one of four e-PTFE grafts) at 30 days, and transmural vasa vasorum were never seen. The seeded endothelial cells migrated rapidly from the sites of initial adhesion near the e-PTFE onto the flow surface. Only one of four of the unseeded e-PTFE grafts had surface endothelium after 30 days, and only moderate coverage developed during 180 days. We conclude that endothelial healing is more rapid m seeded e-PTFE grafts than in seeded Dacron grafts and occurs by a different mechanism.

AB - To detect cellular differences in the healing of polytetrafluoroethylene (e-PTFE) and Dacron grafts up to 7 months after implantation, we studied 108 aortic graft interpositions in dogs. Each prosthesis was alternately prepared by endothelial seeding or by an unseeded control method. The grafts were perfusion fixed and studied with light, scanning, and transmission electron microscopy at intervals from before to 221 days after implantation. Seeding resulted in the development of an extensive endothelial flow surface in two out of three of the e-PTFE and none out of four of the Dacron grafts by 10 days after implantation (p = 0.053). After 30 days a microfibrillar subendothelial matrix ranging from 5 to 11 μ formed in all but three grafts with endothelial coverage. The inner capsule of mature Dacron grafts was significantly thicker (169 ± 143 μ) than in e-PTFE grafts (22 ± 32 μ; p = 0.002). Seeded and unseeded Dacron grafts had predominantly fibroblasts in the outer capsule of the graft by 10 days. Surface endothelium, vasa vasorum, fibroblasts, and myointimal cells appeared in the inner capsule between 10 and 30 days after implantation. In Dacron grafts, fibroblasts and myointimal cells predominated in the inner capsule at 30 days, with smooth muscle cells not being definitely identifiable until after 150 days. Neither fibroblasts nor myointimal cells were common (present but sparce in one of four e-PTFE grafts) at 30 days, and transmural vasa vasorum were never seen. The seeded endothelial cells migrated rapidly from the sites of initial adhesion near the e-PTFE onto the flow surface. Only one of four of the unseeded e-PTFE grafts had surface endothelium after 30 days, and only moderate coverage developed during 180 days. We conclude that endothelial healing is more rapid m seeded e-PTFE grafts than in seeded Dacron grafts and occurs by a different mechanism.

UR - http://www.scopus.com/inward/record.url?scp=0021130739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021130739&partnerID=8YFLogxK

M3 - Article

C2 - 6237448

AN - SCOPUS:0021130739

VL - 96

SP - 745

EP - 755

JO - Surgery

JF - Surgery

SN - 0039-6060

IS - 4

ER -