Endothelial viability of organ-cultured corneas following penetrating keratoplasty

C. L. Harper, M. E. Boulton, B. Marcyniuk, A. B. Tullo, A. E. Ridgway

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose. To assess endothelial integrity following corneal transplantation using human corneas stored in organ culture in the Manchester Eye Bank. Methods. A prospective study was undertaken on 24 patients who had received full-thickness corneal grafts using corneas stored in organ culture. The donor corneal endothelium was photographed prior to transplantation using light microscopy. Specular microscopy and ultrasonic pachometry were performed at 30 days (± 3 days), 12 weeks (± 1 week), 26 weeks (± 2 weeks) and 52 weeks (± 4 weeks) following corneal transplantation. The following cell parameters were measured: density, area, coefficient of variation (CV) for area, perimeter, diameter, form factor and corneal thickness. Results. One year after corneal transplantation there was a statistically significant decrease in endothelial cell density (-39.4%), and a statistically significant increase in endothelial cell area (+94.4%), perimeter (+36.1%), diameter (+57.1%) and form factor (+5.8%). However, no significant changes were seen in CV or corneal thickness with respect to time after transplantation. (There were no significant changes in endothelial cell parameters and corneal thickness for 12 control subjects.) Conclusions. Endothelial cell loss occurs at an accelerated rate from corneal transplants. This highlights the need for improving corneal endothelial viability during and after storage in order to improve the chances of longer-term survival of the transplanted cornea.

Original languageEnglish (US)
Pages (from-to)834-838
Number of pages5
JournalEye (Basingstoke)
Volume12
Issue number5
StatePublished - Oct 1998
Externally publishedYes

Fingerprint

Tissue Survival
Penetrating Keratoplasty
Cornea
Corneal Transplantation
Endothelial Cells
Organ Culture Techniques
Microscopy
Transplantation
Eye Banks
Transplants
Corneal Endothelium
Ultrasonics
Cell Count
Tissue Donors
Prospective Studies
Light
Survival

Keywords

  • Corneal transplantation
  • Endothelium
  • Human
  • Organ culture
  • Pachometry
  • Specular microscopy

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Harper, C. L., Boulton, M. E., Marcyniuk, B., Tullo, A. B., & Ridgway, A. E. (1998). Endothelial viability of organ-cultured corneas following penetrating keratoplasty. Eye (Basingstoke), 12(5), 834-838.

Endothelial viability of organ-cultured corneas following penetrating keratoplasty. / Harper, C. L.; Boulton, M. E.; Marcyniuk, B.; Tullo, A. B.; Ridgway, A. E.

In: Eye (Basingstoke), Vol. 12, No. 5, 10.1998, p. 834-838.

Research output: Contribution to journalArticle

Harper, CL, Boulton, ME, Marcyniuk, B, Tullo, AB & Ridgway, AE 1998, 'Endothelial viability of organ-cultured corneas following penetrating keratoplasty', Eye (Basingstoke), vol. 12, no. 5, pp. 834-838.
Harper CL, Boulton ME, Marcyniuk B, Tullo AB, Ridgway AE. Endothelial viability of organ-cultured corneas following penetrating keratoplasty. Eye (Basingstoke). 1998 Oct;12(5):834-838.
Harper, C. L. ; Boulton, M. E. ; Marcyniuk, B. ; Tullo, A. B. ; Ridgway, A. E. / Endothelial viability of organ-cultured corneas following penetrating keratoplasty. In: Eye (Basingstoke). 1998 ; Vol. 12, No. 5. pp. 834-838.
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abstract = "Purpose. To assess endothelial integrity following corneal transplantation using human corneas stored in organ culture in the Manchester Eye Bank. Methods. A prospective study was undertaken on 24 patients who had received full-thickness corneal grafts using corneas stored in organ culture. The donor corneal endothelium was photographed prior to transplantation using light microscopy. Specular microscopy and ultrasonic pachometry were performed at 30 days (± 3 days), 12 weeks (± 1 week), 26 weeks (± 2 weeks) and 52 weeks (± 4 weeks) following corneal transplantation. The following cell parameters were measured: density, area, coefficient of variation (CV) for area, perimeter, diameter, form factor and corneal thickness. Results. One year after corneal transplantation there was a statistically significant decrease in endothelial cell density (-39.4{\%}), and a statistically significant increase in endothelial cell area (+94.4{\%}), perimeter (+36.1{\%}), diameter (+57.1{\%}) and form factor (+5.8{\%}). However, no significant changes were seen in CV or corneal thickness with respect to time after transplantation. (There were no significant changes in endothelial cell parameters and corneal thickness for 12 control subjects.) Conclusions. Endothelial cell loss occurs at an accelerated rate from corneal transplants. This highlights the need for improving corneal endothelial viability during and after storage in order to improve the chances of longer-term survival of the transplanted cornea.",
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N2 - Purpose. To assess endothelial integrity following corneal transplantation using human corneas stored in organ culture in the Manchester Eye Bank. Methods. A prospective study was undertaken on 24 patients who had received full-thickness corneal grafts using corneas stored in organ culture. The donor corneal endothelium was photographed prior to transplantation using light microscopy. Specular microscopy and ultrasonic pachometry were performed at 30 days (± 3 days), 12 weeks (± 1 week), 26 weeks (± 2 weeks) and 52 weeks (± 4 weeks) following corneal transplantation. The following cell parameters were measured: density, area, coefficient of variation (CV) for area, perimeter, diameter, form factor and corneal thickness. Results. One year after corneal transplantation there was a statistically significant decrease in endothelial cell density (-39.4%), and a statistically significant increase in endothelial cell area (+94.4%), perimeter (+36.1%), diameter (+57.1%) and form factor (+5.8%). However, no significant changes were seen in CV or corneal thickness with respect to time after transplantation. (There were no significant changes in endothelial cell parameters and corneal thickness for 12 control subjects.) Conclusions. Endothelial cell loss occurs at an accelerated rate from corneal transplants. This highlights the need for improving corneal endothelial viability during and after storage in order to improve the chances of longer-term survival of the transplanted cornea.

AB - Purpose. To assess endothelial integrity following corneal transplantation using human corneas stored in organ culture in the Manchester Eye Bank. Methods. A prospective study was undertaken on 24 patients who had received full-thickness corneal grafts using corneas stored in organ culture. The donor corneal endothelium was photographed prior to transplantation using light microscopy. Specular microscopy and ultrasonic pachometry were performed at 30 days (± 3 days), 12 weeks (± 1 week), 26 weeks (± 2 weeks) and 52 weeks (± 4 weeks) following corneal transplantation. The following cell parameters were measured: density, area, coefficient of variation (CV) for area, perimeter, diameter, form factor and corneal thickness. Results. One year after corneal transplantation there was a statistically significant decrease in endothelial cell density (-39.4%), and a statistically significant increase in endothelial cell area (+94.4%), perimeter (+36.1%), diameter (+57.1%) and form factor (+5.8%). However, no significant changes were seen in CV or corneal thickness with respect to time after transplantation. (There were no significant changes in endothelial cell parameters and corneal thickness for 12 control subjects.) Conclusions. Endothelial cell loss occurs at an accelerated rate from corneal transplants. This highlights the need for improving corneal endothelial viability during and after storage in order to improve the chances of longer-term survival of the transplanted cornea.

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