Endothelin-1 enhances capsaicin-induced peptide release and cGMP accumulation in cultures of rat sensory neurons

Julia Dymshitz, Michael R. Vasko

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Because endothelin-1 (ET-1) may be a neuromodulator in sensory systems, we examined whether this peptide could alter release of substance P (SP) and calcitonin gene-related peptide (CGRP) from isolated sensory neurons. Although ET-1 had minimal actions on spontaneous neuropeptide release, pretreating cultures with 500 nM resulted in a 50% augmentation of SP and CGRP release evoked by 50 nM capsaicin. Moreover, 2000 nM ET-1 enhanced capsaicin-evoked release of CGRP two fold. In an analogous manner, ET-1 alone did not alter intracellular cGMP content, but enhanced the increase in cGMP caused by 50 nM capsaicin. Intracellular cAMP was not altered by capsaicin and/or ET-1. These data suggest that ET-1 may play a role in modulation of peptide release from primary afferent neurons.

Original languageEnglish (US)
Pages (from-to)128-132
Number of pages5
JournalNeuroscience Letters
Volume167
Issue number1-2
DOIs
StatePublished - Feb 14 1994

Keywords

  • Calcitonin gene-related peptide
  • Capsaicin
  • cGMP
  • Dorsal root ganglion
  • Endothelin-1
  • Peptide release
  • substance P

ASJC Scopus subject areas

  • Neuroscience(all)

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