Endothelin antagonism does not affect adrenal catecholamine secretion and system haemodynamics during acute stress in Wistar rats

D. V. Zaretsky, M. V. Zaretskaia, E. I. Kalenikova, M. A. Grafov

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3 Scopus citations


In the present work we evaluated the role of circulating endothelin-1 (ET-1) in haemodynamics and the adrenal medulla responses to stress using PD-142893, a mixed endothelin-A- and -B- (ET(A)/ET(B)) receptor antagonist. Male Wistar rats were chronically instrumented with arterial and venous catheters and a microdialysis probe placed into the adrenal medulla. Stress was induced by a 1 h period of immobilization. PD-142893 was infused (20 μg/kg/min) for 90 min starting 30 min before stress onset. Concentrations of norepinephrine and epinephrine in dialysate samples were measured by high-performance liquid chromatography (HPLC). At rest animals displayed a stable level of norepinephrine (85 ± 34 pg/ml) and epinephrine (176 ± 57 pg/ml) in adrenal perfusate and constant haemodynamic parameters. Stress increased adrenal secretion (norepinephrine 206 ± 50%, epinephrine 202 ± 45%) associated with hypertension (peak 141 ± 3 mmHg) and tachycardia (peak 505 ± 5 bpm). No significant changes in haemodynamics or of plasma catecholamine levels were observed during infusion of PD-142893. The antagonist did not significantly change the stress-induced increase in catecholamine secretion, tachycardia or hypertension. Thus, in Wistar rats, circulating ET-1 seems not to be essential for blood pressure control or adrenal catecholamine secretion during acute stress.

Original languageEnglish (US)
Pages (from-to)S374-S376
JournalJournal of cardiovascular pharmacology
Issue number5 SUPPL. 1
StatePublished - Jan 1 2000



  • Adrenal medulla
  • Cardiovascular system
  • Catecholamines
  • Endothelin
  • Rats

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

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