Endotoxin enhances tissue factor and suppresses thrombomodulin expression of human vascular endothelium in vitro

K. L. Moore, Sharon Andreoli, N. L. Esmon, N. U. Bang

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Abstract

Endotoxemia is frequently associated clinically with disseminated intravascular coagulation (DIC); however, the mechanism of endotoxin action in vivo is unclear. Modulation of tissue factor (TF) and thrombomodulin (TM) expression on the endothelial surface may be relevant pathophysiologic mechanisms. Stimulation of human umbilical vein endothelial cells with endotoxin (1 μg/ml) increased surface TF activity from 1.52 ± 0.84 to 11.89 ± 8.12 mU/ml-106 cells at 6 h (n = 11) which returned to baseline by 24 h. Repeated stimulation at 24 h resulted in renewed TF expression. Endotoxin (1 μg/ml) also caused a decrease in TM expression to 55.0 ± 6.4% of control levels at 24 h (n = 10) that remained depressed at 48 h. Both effects were dose and serum dependent. A temporary rise in TF expression accompanied by a sustained fall in TM expression comprise a shift in the hemostatic properties of the endothelium that would favor intravascular coagulation and may contribute to the pathogenesis of DIC in gram-negative septicemia.

Original languageEnglish
Pages (from-to)124-130
Number of pages7
JournalJournal of Clinical Investigation
Volume79
Issue number1
StatePublished - 1987

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Vascular Endothelium
Thromboplastin
Thrombomodulin
Endotoxins
Disseminated Intravascular Coagulation
Endotoxemia
Human Umbilical Vein Endothelial Cells
Hemostatics
Endothelium
Sepsis
In Vitro Techniques
human THBD protein
Serum

ASJC Scopus subject areas

  • Medicine(all)

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Endotoxin enhances tissue factor and suppresses thrombomodulin expression of human vascular endothelium in vitro. / Moore, K. L.; Andreoli, Sharon; Esmon, N. L.; Bang, N. U.

In: Journal of Clinical Investigation, Vol. 79, No. 1, 1987, p. 124-130.

Research output: Contribution to journalArticle

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