Endotoxin provocation of experimental renal cystic disease

K. D. Gardner, W. P. Reed, Andrew Evan, J. Zedalis, M. D. Hylarides, A. A. Leon

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Germ free rats provide a unique model in which to assess biological response to environment. In 48 germ-free male, Sprague-Dawley rats we examined the consequences of oral exposure to nordihydroguaiaretic acid (NDGA), a nephrotoxin; to Staphylococcus epidermidis and bacillus species, non-endotoxin-containing bacteria; to Escherichia coli and Proteus mirabilis, endotoxin-containing bacteria; and injected E. coli endotoxin on peripheral leukocyte counts and renal morphology. Morphological changes were evaluated by light microscopy and scored blindly on a 0 to 4+ scale for 15 parameters of renal structure. Means of these renal 'pathology scores' correlated with counts of polymorphonuclear leukocytes and lymphocytes in the peripheral blood. The highest counts and scores were found in rats given NDGA and exposed to endotoxin, either by injection or by oral feeding of endotoxin-containing bacteria. Counts and scores were lower in the absence of endotoxin and with non-endotoxin-containing bacteria, given alone or in combination with either NDGA or endotoxin. Results exclude bacterial colonization and intrarenal accumulation of NDGA as causes of nephropathy. They indicate that endotoxin and NDGA act synergistically to provoke renal damage in the germ free NDGA-fed rat and suggest that leukocytes are involved in the process.

Original languageEnglish (US)
Pages (from-to)329-334
Number of pages6
JournalKidney International
Volume32
Issue number3
StatePublished - 1987
Externally publishedYes

Fingerprint

Masoprocol
Cystic Kidney Diseases
Endotoxins
Bacteria
Kidney
Proteus mirabilis
Staphylococcus epidermidis
Lymphocyte Count
Leukocyte Count
Bacillus
Sprague Dawley Rats
Microscopy
Neutrophils
Leukocytes
Pathology
Escherichia coli
Light
Injections

ASJC Scopus subject areas

  • Nephrology

Cite this

Gardner, K. D., Reed, W. P., Evan, A., Zedalis, J., Hylarides, M. D., & Leon, A. A. (1987). Endotoxin provocation of experimental renal cystic disease. Kidney International, 32(3), 329-334.

Endotoxin provocation of experimental renal cystic disease. / Gardner, K. D.; Reed, W. P.; Evan, Andrew; Zedalis, J.; Hylarides, M. D.; Leon, A. A.

In: Kidney International, Vol. 32, No. 3, 1987, p. 329-334.

Research output: Contribution to journalArticle

Gardner, KD, Reed, WP, Evan, A, Zedalis, J, Hylarides, MD & Leon, AA 1987, 'Endotoxin provocation of experimental renal cystic disease', Kidney International, vol. 32, no. 3, pp. 329-334.
Gardner KD, Reed WP, Evan A, Zedalis J, Hylarides MD, Leon AA. Endotoxin provocation of experimental renal cystic disease. Kidney International. 1987;32(3):329-334.
Gardner, K. D. ; Reed, W. P. ; Evan, Andrew ; Zedalis, J. ; Hylarides, M. D. ; Leon, A. A. / Endotoxin provocation of experimental renal cystic disease. In: Kidney International. 1987 ; Vol. 32, No. 3. pp. 329-334.
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