Abstract
Background: Aerobic exercise has been shown to slow tumor progression in rodents and humans, but the mechanisms behind this effect are still unclear. Here we show that aerobic exercise in the form of chronic endurance training suppresses tumor recruitment of FoxP3+ Treg cells thus enhancing antitumor immune efficiency. Methods: Adult wild-type and athymic BALB/c female mice were endurance-trained for 8 weeks. Circulating leukocytes as well as muscle and liver mtDNA copy number were compared to aged-matched concurrent sedentary controls to establish systemic effects. 4 T1 murine mammary tumor cells were injected subcutaneously to the 4th mammary pad at the end of the training period. Tumor growth and survival rates were compared, together with antitumor immune response. Results: Exercised wild-type had 17% slower growth rate, 24% longer survival, and 2-fold tumor-CD+ 8/FoxP3+ ratio than sedentary controls. Exercised athymic BALB/c females showed no difference in tumor growth or survival rates when compared to sedentary controls. Conclusions: Cytotoxic T cells are a significant factor in endurance exercise-induced suppression of tumor growth. Endurance exercise enhances antitumor immune efficacy by increasing intratumoral CD8+/FoxP3+ ratio.
| Original language | English (US) |
|---|---|
| Article number | 536 |
| Journal | BMC Cancer |
| Volume | 19 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jun 4 2019 |
Fingerprint
Keywords
- CD8/FoxP3 ratio
- Endurance exercise
- Forced running wheels
- Hypoxia
- Murine mammary tumor
- Solid tumor progression
- Treg cells
ASJC Scopus subject areas
- Oncology
- Genetics
- Cancer Research
Cite this
Endurance training slows breast tumor growth in mice by suppressing Treg cells recruitment to tumors. / Hagar, Amit; Wang, Zemin; Koyama, Sachiko; Serrano, Josua Aponte; Melo, Luma; Vargas, Stephanie; Carpenter, Richard; Foley, John.
In: BMC Cancer, Vol. 19, No. 1, 536, 04.06.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Endurance training slows breast tumor growth in mice by suppressing Treg cells recruitment to tumors
AU - Hagar, Amit
AU - Wang, Zemin
AU - Koyama, Sachiko
AU - Serrano, Josua Aponte
AU - Melo, Luma
AU - Vargas, Stephanie
AU - Carpenter, Richard
AU - Foley, John
PY - 2019/6/4
Y1 - 2019/6/4
N2 - Background: Aerobic exercise has been shown to slow tumor progression in rodents and humans, but the mechanisms behind this effect are still unclear. Here we show that aerobic exercise in the form of chronic endurance training suppresses tumor recruitment of FoxP3+ Treg cells thus enhancing antitumor immune efficiency. Methods: Adult wild-type and athymic BALB/c female mice were endurance-trained for 8 weeks. Circulating leukocytes as well as muscle and liver mtDNA copy number were compared to aged-matched concurrent sedentary controls to establish systemic effects. 4 T1 murine mammary tumor cells were injected subcutaneously to the 4th mammary pad at the end of the training period. Tumor growth and survival rates were compared, together with antitumor immune response. Results: Exercised wild-type had 17% slower growth rate, 24% longer survival, and 2-fold tumor-CD+ 8/FoxP3+ ratio than sedentary controls. Exercised athymic BALB/c females showed no difference in tumor growth or survival rates when compared to sedentary controls. Conclusions: Cytotoxic T cells are a significant factor in endurance exercise-induced suppression of tumor growth. Endurance exercise enhances antitumor immune efficacy by increasing intratumoral CD8+/FoxP3+ ratio.
AB - Background: Aerobic exercise has been shown to slow tumor progression in rodents and humans, but the mechanisms behind this effect are still unclear. Here we show that aerobic exercise in the form of chronic endurance training suppresses tumor recruitment of FoxP3+ Treg cells thus enhancing antitumor immune efficiency. Methods: Adult wild-type and athymic BALB/c female mice were endurance-trained for 8 weeks. Circulating leukocytes as well as muscle and liver mtDNA copy number were compared to aged-matched concurrent sedentary controls to establish systemic effects. 4 T1 murine mammary tumor cells were injected subcutaneously to the 4th mammary pad at the end of the training period. Tumor growth and survival rates were compared, together with antitumor immune response. Results: Exercised wild-type had 17% slower growth rate, 24% longer survival, and 2-fold tumor-CD+ 8/FoxP3+ ratio than sedentary controls. Exercised athymic BALB/c females showed no difference in tumor growth or survival rates when compared to sedentary controls. Conclusions: Cytotoxic T cells are a significant factor in endurance exercise-induced suppression of tumor growth. Endurance exercise enhances antitumor immune efficacy by increasing intratumoral CD8+/FoxP3+ ratio.
KW - CD8/FoxP3 ratio
KW - Endurance exercise
KW - Forced running wheels
KW - Hypoxia
KW - Murine mammary tumor
KW - Solid tumor progression
KW - Treg cells
UR - http://www.scopus.com/inward/record.url?scp=85066843497&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85066843497&partnerID=8YFLogxK
U2 - 10.1186/s12885-019-5745-7
DO - 10.1186/s12885-019-5745-7
M3 - Article
C2 - 31164094
AN - SCOPUS:85066843497
VL - 19
JO - BMC Cancer
JF - BMC Cancer
SN - 1471-2407
IS - 1
M1 - 536
ER -