Engineering productive enzyme confinement

A. Keith Dunker, Ariel Fernández

Research output: Contribution to journalShort survey

19 Scopus citations


Natural enzymes were selected to function inside the cell, not in the test tube; therefore, their performance is optimized for the crowded conditions encountered in vivo. Most man-made matrices for enzyme confinement lead to suboptimal catalytic activity. Ackerman and colleagues showed that an entrapping environment consisting of functionalized mesoporous silica actually enhances enzyme activity beyond the test-tube levels of free enzymes in solution. These findings provide an approach for dissecting the effect of various contributors to enzyme activity and thereby provide a means for fine-tuning the entrapping matrices to optimize enzyme performance in a rational way.

Original languageEnglish (US)
Pages (from-to)189-190
Number of pages2
JournalTrends in Biotechnology
Issue number5
StatePublished - May 1 2007

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

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