Engineering productive enzyme confinement

A. Dunker, Ariel Fernández

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Natural enzymes were selected to function inside the cell, not in the test tube; therefore, their performance is optimized for the crowded conditions encountered in vivo. Most man-made matrices for enzyme confinement lead to suboptimal catalytic activity. Ackerman and colleagues showed that an entrapping environment consisting of functionalized mesoporous silica actually enhances enzyme activity beyond the test-tube levels of free enzymes in solution. These findings provide an approach for dissecting the effect of various contributors to enzyme activity and thereby provide a means for fine-tuning the entrapping matrices to optimize enzyme performance in a rational way.

Original languageEnglish
Pages (from-to)189-190
Number of pages2
JournalTrends in Biotechnology
Volume25
Issue number5
DOIs
StatePublished - May 2007

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Enzymes
Enzyme activity
Silicon Dioxide
Catalyst activity
Tuning
Silica

ASJC Scopus subject areas

  • Bioengineering

Cite this

Engineering productive enzyme confinement. / Dunker, A.; Fernández, Ariel.

In: Trends in Biotechnology, Vol. 25, No. 5, 05.2007, p. 189-190.

Research output: Contribution to journalArticle

Dunker, A. ; Fernández, Ariel. / Engineering productive enzyme confinement. In: Trends in Biotechnology. 2007 ; Vol. 25, No. 5. pp. 189-190.
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