Enhanced adiponectin multimer ratio and skeletal muscle adiponectin receptor expression following exercise training and diet in older insulin-resistant adults

Valerie B. O'Leary, Ashley E. Jorett, Christine M. Marchetti, Frank Gonzalez, Susan A. Phillips, Theodore P. Ciaraldi, John P. Kirwan

Research output: Contribution to journalArticle

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Abstract

Circulating adiponectin is reduced in disorders associated with insulin resistance. This study was conducted to determine whether an exercise/diet intervention would alter adiponectin multimer distribution and adiponectin receptor expression in skeletal muscle. Impaired glucose-tolerant older (>60 yr) obese (BMI 30-40 kg/m2) men (n = 7) and women (n = 14) were randomly assigned to 12 wk of supervised aerobic exercise combined with either a hypocaloric (ExHypo, ∼500 kcal reduction, n = 11) or eucaloric diet (ExEu, n = 10). Insulin sensitivity was determined by the euglycemic (5.0 mM) hyperinsulinemic (40 mU·m-2·min-1) clamp. Adiponectin multimers [high (HMW), middle (MMW), and low molecular weight (LMW)] were measured by nondenaturing Western blot analysis. Relative quantification of adiponectin receptor expression through RT-PCR was determined from skeletal muscle biopsy samples. Greater weight loss occurred in ExHypo compared with ExEu subjects (8.0 ± 0.6 vs. 3.2 ± 0.6%, P <0.0001). Insulin sensitivity improved postintervention in both groups (ExHypo: 2.5 ± 0.3 vs. 4.4 ± 0.5 mg·kg FFM-1·min-1, and ExEu: 2.9 ± 0.4 vs. 4.1 ± 0.4 mg·kg FFM -1·min-1, P <0.0001). Comparison of multimer isoforms revealed a decreased percentage in MMW relative to HMW and LMW (P <0.03). The adiponectin SA ratio (HMW/total) was increased following both interventions (P <0.05) and correlated with the percent change in insulin sensitivity (P <0.03). Postintervention adiponectin receptor mRNA expression was also significantly increased (AdipoR1 P <0.03, AdipoR2 P <0.02). These data suggest that part of the improvement in insulin sensitivity following exercise and diet may be due to changes in the adiponectin oligomeric distribution and enhanced membrane receptor expression.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume293
Issue number1
DOIs
StatePublished - Jul 2007
Externally publishedYes

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Adiponectin Receptors
Adiponectin
Nutrition
Muscle
Insulin Resistance
Skeletal Muscle
Exercise
Insulin
Diet
Molecular Weight
Molecular weight
Biopsy
Clamping devices
Weight Loss
Protein Isoforms
Western Blotting
Membranes
Glucose
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Aging
  • Diabetes
  • Gene expression
  • Glucose intolerance
  • Obesity

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry

Cite this

Enhanced adiponectin multimer ratio and skeletal muscle adiponectin receptor expression following exercise training and diet in older insulin-resistant adults. / O'Leary, Valerie B.; Jorett, Ashley E.; Marchetti, Christine M.; Gonzalez, Frank; Phillips, Susan A.; Ciaraldi, Theodore P.; Kirwan, John P.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 293, No. 1, 07.2007.

Research output: Contribution to journalArticle

O'Leary, Valerie B. ; Jorett, Ashley E. ; Marchetti, Christine M. ; Gonzalez, Frank ; Phillips, Susan A. ; Ciaraldi, Theodore P. ; Kirwan, John P. / Enhanced adiponectin multimer ratio and skeletal muscle adiponectin receptor expression following exercise training and diet in older insulin-resistant adults. In: American Journal of Physiology - Endocrinology and Metabolism. 2007 ; Vol. 293, No. 1.
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