Enhanced autophagy signaling in diabetic rats with ischemia-induced seizures

Luoxing Xia, Zhigang Lei, Zhongshan Shi, Dave Guo, Henry Su, Yiwen Ruan, Zao C. Xu

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Seizures are among the most common neurological sequelae of stroke, and ischemic insult in diabetes notably increases the incidence of seizures. Recent studies indicated that autophagy influences the outcome of stroke and involved in epileptogenesis. However, the association of autophagy and post-ischemic seizures in diabetes remains unclear. The present study aimed to reveal the involvement of autophagy in the seizures following cerebral ischemia in diabetes. Diabetes was induced in adult male Wistar rats by intraperitoneal injection of streptozotocin (STZ). The diabetic rats were subjected to transient forebrain ischemia. The neuronal damage was assessed using hematoxylin-eosin staining. Western blotting and immunohistochemistry were performed to investigate the alteration of autophagy marker microtubule-associated protein light chain 1B (LC3B). The results showed that all diabetic animals developed seizures after ischemia. However, no apparent cell death was observed in the hippocampus of seizure rats 12 h after the insult. The expression of LC3B was significantly enhanced in naïve animals after ischemia and was further increased in diabetic animals after ischemia. Immunofluorescence double-labeling study indicated that LC3B was mainly increased in neurons. Our study demonstrated, for the first time, that autophagy activity is significantly increased in diabetic animals with ischemia-induced seizures. Further studies are needed to explore the role of autophagy in seizure generation after ischemia in diabetic conditions.

Original languageEnglish (US)
Pages (from-to)18-26
Number of pages9
JournalBrain Research
Volume1643
DOIs
StatePublished - Jul 15 2016

Keywords

  • Epilepsy
  • Hippocampus
  • Hyperglycemia
  • LC3
  • Stroke

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

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  • Cite this

    Xia, L., Lei, Z., Shi, Z., Guo, D., Su, H., Ruan, Y., & Xu, Z. C. (2016). Enhanced autophagy signaling in diabetic rats with ischemia-induced seizures. Brain Research, 1643, 18-26. https://doi.org/10.1016/j.brainres.2016.04.054