Enhanced collection of phenotypic and engrafting human cord blood hematopoietic stem cells at 4°C

Hal E. Broxmeyer, Scott Cooper, Maegan L. Capitano

Research output: Contribution to journalArticle

Abstract

Hematopoietic stem cell (HSC) numbers collected in cord blood (CB) at the birth of a baby is a limiting factor for efficacious use of CB in hematopoietic cell transplantation (HCT). We now demonstrate that collecting and processing of human CB at 4°C within minutes of the baby's birth results in significantly enhanced numbers of rigorously defined phenotypic HSC and self-renewing NSG immune-deficient mouse engrafting and SCID-repopulating cells. This was associated with decreased numbers of hematopoietic progenitor cells (HPC), as noted previously for hypoxia collected/processed cells blocking ambient air induced differentiation of HSC to HPC. We have thus defined a simple, cost-effective, means to collect increased numbers of CB HSC, of potential use for clinical CB HCT.

Original languageEnglish (US)
JournalSTEM CELLS
DOIs
StateAccepted/In press - 2020

Keywords

  • cord blood
  • hematopoietic cell transplantation
  • hematopoietic progenitor cells
  • hematopoietic stem cells

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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