Enhanced erbB-3 Expression in Human Pancreatic Cancer Correlates with Tumor Progression

Helmut Friess, Yoichiro Yamanaka, Michael S. Kobrin, David A. Do, Markus W. Büchler, Murray Korc

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Abstract

The erbB-3 gene encodes a transmembrane protein that is related to the epidermal growth factor (EGF) receptor and erbB-2. We compared erbB-3 expression in the normal human pancreas, human pancreatic carcinomas, and cultured human pancreatic cancer cell lines. Northern blot analysis of total RNA revealed the anticipated 6.2-kb mRNA transcript in all 19 normal pancreatic samples. In 17 of 27 pancreatic cancers, there was a 6.7-fold increase (P < 0.001) in erbB-3 mRNA levels. Southern blot analysis did not reveal erbB-3 gene amplification. Four of six pancreatic cancer cell lines exhibited the 6.2-kb erbB-3 mRNA transcript, and all four cell lines coexpressed the epidermal growth factor receptor and erbB-2. Using a highly specific antibody, we determined that faint to moderate erbB-3 immunoreactivity was present in the ductal cells in the normal pancreas. In 47% (27/58) of the pancreatic cancers, there were many cancer cells with intense erbB-3 immunostaining. The presence of erbB-3 in the cancer cells was associated with advanced tumor stage and shorter survival postoperatively. These data indicate that a significant proportion of human pancreatic cancers overexpress erbB-3, and that erbB-3 may contribute to disease progression in this disorder.

Original languageEnglish (US)
Pages (from-to)1413-1420
Number of pages8
JournalClinical Cancer Research
Volume1
Issue number11
StatePublished - Nov 1 1995

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Friess, H., Yamanaka, Y., Kobrin, M. S., Do, D. A., Büchler, M. W., & Korc, M. (1995). Enhanced erbB-3 Expression in Human Pancreatic Cancer Correlates with Tumor Progression. Clinical Cancer Research, 1(11), 1413-1420.