Enhanced erbB-3 expression in human pancreatic cancer correlates with tumor progression

H. Friess, Y. Yamanaka, M. S. Kobrin, D. A. Do, M. W. Buchler, Murray Korc

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

The erbB-3 gene encodes a transmembrane protein that is related to the epidermal growth factor (EGF) receptor and erbB-2. We compared erbB-3 expression in the normal human pancreas, human pancreatic carcinomas, and cultured human pancreatic cancer cell lines. Northern blot analysis of total RNA revealed the anticipated 6.2-kb mRNA transcript in all 19 normal pancreatic samples. In 17 of 27 pancreatic cancers, there was a 6.7-fold increase (P <0.001) in erbB-3 mRNA levels. Southern blot analysis did not reveal erbB-3 gene amplification. Four of six pancreatic cancer cell lines exhibited the 6.2-kb erbB-3 mRNA transcript, and all four cell lines coexpressed the epidermal growth factor receptor and erbB-2. Using a highly specific antibody, we determined that faint to moderate erbB-3 immunoreactivity was present in the ductal cells in the normal pancreas. In 47% (27/58) of the pancreatic cancers, there were many cancer cells with intense erbB-3 immunostaining. The presence of erbB-3 in the cancer cells was associated with advanced tumor stage and shorter survival postoperatively. These data indicate that a significant proportion of human pancreatic cancers overexpress erbB-3, and that erbB-3 may contribute to disease progression in this disorder.

Original languageEnglish (US)
Pages (from-to)1413-1420
Number of pages8
JournalClinical Cancer Research
Volume1
Issue number11
StatePublished - 1995
Externally publishedYes

Fingerprint

Pancreatic Neoplasms
erbB Genes
Neoplasms
Epidermal Growth Factor Receptor
Cell Line
Messenger RNA
Pancreas
Gene Amplification
Southern Blotting
Northern Blotting
Disease Progression
RNA
Survival
Antibodies
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Friess, H., Yamanaka, Y., Kobrin, M. S., Do, D. A., Buchler, M. W., & Korc, M. (1995). Enhanced erbB-3 expression in human pancreatic cancer correlates with tumor progression. Clinical Cancer Research, 1(11), 1413-1420.

Enhanced erbB-3 expression in human pancreatic cancer correlates with tumor progression. / Friess, H.; Yamanaka, Y.; Kobrin, M. S.; Do, D. A.; Buchler, M. W.; Korc, Murray.

In: Clinical Cancer Research, Vol. 1, No. 11, 1995, p. 1413-1420.

Research output: Contribution to journalArticle

Friess, H, Yamanaka, Y, Kobrin, MS, Do, DA, Buchler, MW & Korc, M 1995, 'Enhanced erbB-3 expression in human pancreatic cancer correlates with tumor progression', Clinical Cancer Research, vol. 1, no. 11, pp. 1413-1420.
Friess H, Yamanaka Y, Kobrin MS, Do DA, Buchler MW, Korc M. Enhanced erbB-3 expression in human pancreatic cancer correlates with tumor progression. Clinical Cancer Research. 1995;1(11):1413-1420.
Friess, H. ; Yamanaka, Y. ; Kobrin, M. S. ; Do, D. A. ; Buchler, M. W. ; Korc, Murray. / Enhanced erbB-3 expression in human pancreatic cancer correlates with tumor progression. In: Clinical Cancer Research. 1995 ; Vol. 1, No. 11. pp. 1413-1420.
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