Enhanced expression of cyclooxygenase-2 in high grade human transitional cell bladder carcinomas

Martin Kömhoff, Youfei Guan, Heidi W. Shappell, Linda Davis, Greg Jack, Yu Shyr, Michael O. Koch, Scott B. Shappell, Matthew D. Breyer

Research output: Contribution to journalArticle

139 Scopus citations

Abstract

Studies in human and animal models have shown that cyclooxygenase (COX)-2 is up-regulated in several epithelial carcinomas including colon, breast, and lung. To elucidate the possible involvement of COX-2 in human bladder cancer we examined the expression of COX isoforms in benign tissue and in bladder carcinoma specimens. Paraffin embedded tissues from 75 patients with urothelial carcinomas were immunostained with specific antibodies raised against COX-1 and COX-2. COX-1 expression was detected in smooth muscle cells in both benign and malignant bladders. COX-2 immunoreactivity was absent in benign tissue and in specimens with low-grade urothelial carcinoma (0/23). In contrast, expression of COX-2 was detected in malignant epithelial cells in 38% (17/47) of specimens with high-grade urothelial carcinomas. Expression of COX-2 in high-grade bladder cancer was confirmed by radioactive in situ hybridization using a COX-2-selective riboprobe. Both immunohistochemistry and in situ hybridization showed COX-2 expression in a small subset of malignant cells. COX-2 mRNA was also expressed in three out of seven malignant urothelial cell lines. These data demonstrate elevated expression of COX-2 in a high percentage of high-grade bladder carcinomas, suggesting a possible role of COX-2 in the progression of bladder urothelial carcinoma and supporting its potential as a therapeutic target in human bladder carcinoma.

Original languageEnglish (US)
Article number64513
Pages (from-to)29-35
Number of pages7
JournalAmerican Journal of Pathology
Volume157
Issue number1
DOIs
StatePublished - Jul 2000

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Enhanced expression of cyclooxygenase-2 in high grade human transitional cell bladder carcinomas'. Together they form a unique fingerprint.

  • Cite this

    Kömhoff, M., Guan, Y., Shappell, H. W., Davis, L., Jack, G., Shyr, Y., Koch, M. O., Shappell, S. B., & Breyer, M. D. (2000). Enhanced expression of cyclooxygenase-2 in high grade human transitional cell bladder carcinomas. American Journal of Pathology, 157(1), 29-35. [64513]. https://doi.org/10.1016/S0002-9440(10)64513-0