Enhanced expression of cyclooxygenase-2 in high grade human transitional cell bladder carcinomas

M. Komhoff, Y. Guan, H. W. Shappell, L. Davis, G. Jack, Y. Shyr, Michael Koch, S. B. Shappell, M. D. Breyer

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Studies in human and animal models have shown that cyclooxygenase (COX)-2 is up-regulated in several epithelial carcinomas including colon, breast, and lung. To elucidate the possible involvement of COX-2 in human bladder cancer we examined the expression of COX isoforms in benign tissue and in bladder carcinoma specimens. Paraffin embedded tissues from 75 patients with urothelial carcinomas were immunostained with specific antibodies raised against COX-1 and COX-2. COX-1 expression was detected in smooth muscle cells in both benign and malignant bladders. COX-2 immunoreactivity was absent in benign tissue and in specimens with low-grade urothelial carcinoma (0/23). In contrast, expression of COX-2 was detected in malignant epithelial cells in 38% (17/47) of specimens with high-grade urothelial carcinomas. Expression of COX-2 in high-grade bladder cancer was confirmed by radioactive in situ hybridization using a COX-2-selective riboprobe. Both immunohistochemistry and in situ hybridization showed COX-2 expression in a small subset of malignant cells. COX-2 mRNA was also expressed in three out of seven malignant urothelial cell lines. These data demonstrate elevated expression of COX-2 in a high percentage of high-grade bladder carcinomas, suggesting a possible role of COX-2 in the progression of bladder urothelial carcinoma and supporting its potential as a therapeutic target in human bladder carcinoma.

Original languageEnglish (US)
Pages (from-to)29-35
Number of pages7
JournalAmerican Journal of Pathology
Volume157
Issue number1
StatePublished - 2000
Externally publishedYes

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Transitional Cell Carcinoma
Cyclooxygenase 2
Urinary Bladder
Carcinoma
Cyclooxygenase 1
Urinary Bladder Neoplasms
In Situ Hybridization
Prostaglandin-Endoperoxide Synthases
Paraffin
Smooth Muscle Myocytes
Protein Isoforms
Colon
Breast
Animal Models
Epithelial Cells
Immunohistochemistry
Cell Line

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Komhoff, M., Guan, Y., Shappell, H. W., Davis, L., Jack, G., Shyr, Y., ... Breyer, M. D. (2000). Enhanced expression of cyclooxygenase-2 in high grade human transitional cell bladder carcinomas. American Journal of Pathology, 157(1), 29-35.

Enhanced expression of cyclooxygenase-2 in high grade human transitional cell bladder carcinomas. / Komhoff, M.; Guan, Y.; Shappell, H. W.; Davis, L.; Jack, G.; Shyr, Y.; Koch, Michael; Shappell, S. B.; Breyer, M. D.

In: American Journal of Pathology, Vol. 157, No. 1, 2000, p. 29-35.

Research output: Contribution to journalArticle

Komhoff, M, Guan, Y, Shappell, HW, Davis, L, Jack, G, Shyr, Y, Koch, M, Shappell, SB & Breyer, MD 2000, 'Enhanced expression of cyclooxygenase-2 in high grade human transitional cell bladder carcinomas', American Journal of Pathology, vol. 157, no. 1, pp. 29-35.
Komhoff M, Guan Y, Shappell HW, Davis L, Jack G, Shyr Y et al. Enhanced expression of cyclooxygenase-2 in high grade human transitional cell bladder carcinomas. American Journal of Pathology. 2000;157(1):29-35.
Komhoff, M. ; Guan, Y. ; Shappell, H. W. ; Davis, L. ; Jack, G. ; Shyr, Y. ; Koch, Michael ; Shappell, S. B. ; Breyer, M. D. / Enhanced expression of cyclooxygenase-2 in high grade human transitional cell bladder carcinomas. In: American Journal of Pathology. 2000 ; Vol. 157, No. 1. pp. 29-35.
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