Enhanced expression of the type II transforming growth factor β receptor in human pancreatic cancer cells without alteration of type III receptor expression

Helmut Friess, Yoichiro Yamanaka, Markus Büchler, Hans G. Beger, Murray Korc, Rae Lynn Baldwin, Murray Korc

Research output: Contribution to journalArticle

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Abstract

We have recently found that human pancreatic adenocarcinomas exhibit strong immunostaining for the three mammalian transforming growth factor β (TGF-β) isoforms. These important growth-regulating polypeptides bind to a number of proteins, including the type I TGF-β receptor (TβR-I), type II TGF-β receptor (TβR-II), and the type III TGF-β receptor (TβR-III). In the present study we sought to determine whether TβR-II and TβR-III expression is altered in pancreatic cancer. Northern blot analysis indicated that, by comparison with the normal pancreas, pancreatic adenocarcinomas exhibited a 4.6-fold increase (P <0.01) in mRNA levels encoding TβR-II. In contrast, mRNA levels encoding TβR-III were not increased. In situ hybridization showed that TβR-II mRNA was expressed in the majority of cancer cells, whereas mRNA grains encoding TβR-III were detectable in only a few cancer cells and were present mainly in the surrounding stroma. These findings suggest that enhanced levels of TβR-II may have a role in regulating human pancreatic cancer cell growth, while TβR-III may function in the extracellular matrix.

Original languageEnglish (US)
Pages (from-to)2704-2707
Number of pages4
JournalCancer Research
Volume53
Issue number12
StatePublished - Jun 15 1993
Externally publishedYes

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Growth Factor Receptors
Transforming Growth Factors
Pancreatic Neoplasms
Messenger RNA
Adenocarcinoma
Growth
Northern Blotting
In Situ Hybridization
Extracellular Matrix
Pancreas
Neoplasms
Protein Isoforms
Peptides
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Friess, H., Yamanaka, Y., Büchler, M., Beger, H. G., Korc, M., Baldwin, R. L., & Korc, M. (1993). Enhanced expression of the type II transforming growth factor β receptor in human pancreatic cancer cells without alteration of type III receptor expression. Cancer Research, 53(12), 2704-2707.

Enhanced expression of the type II transforming growth factor β receptor in human pancreatic cancer cells without alteration of type III receptor expression. / Friess, Helmut; Yamanaka, Yoichiro; Büchler, Markus; Beger, Hans G.; Korc, Murray; Baldwin, Rae Lynn; Korc, Murray.

In: Cancer Research, Vol. 53, No. 12, 15.06.1993, p. 2704-2707.

Research output: Contribution to journalArticle

Friess, H, Yamanaka, Y, Büchler, M, Beger, HG, Korc, M, Baldwin, RL & Korc, M 1993, 'Enhanced expression of the type II transforming growth factor β receptor in human pancreatic cancer cells without alteration of type III receptor expression', Cancer Research, vol. 53, no. 12, pp. 2704-2707.
Friess, Helmut ; Yamanaka, Yoichiro ; Büchler, Markus ; Beger, Hans G. ; Korc, Murray ; Baldwin, Rae Lynn ; Korc, Murray. / Enhanced expression of the type II transforming growth factor β receptor in human pancreatic cancer cells without alteration of type III receptor expression. In: Cancer Research. 1993 ; Vol. 53, No. 12. pp. 2704-2707.
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