Enhanced expression of transforming growth factor β isoforms in pancreatic cancer correlates with decreased survival

Helmut Friess, Yoichiro Yamanaka, Markus Büchler, Matthias Ebert, Hans G. Beger, Leslie I. Gold, Murray Korc

Research output: Contribution to journalArticle

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Abstract

Background: Transforming growth factor βs (TGF-βs) constitute a family of bifunctional polypeptide growth factors that either inhibit or stimulate cell proliferation. Perturbations in TGF-β expression and function may lead to loss of negative constraints on cell growth. In this study, we examined TGF-β expression in human pancreatic cancer. Methods: The distribution of TGF-β isoforms in 60 human pancreatic cancers was examined using immunohistochemical, Northern blot, and in situ hybridization techniques. Results: Immunohistochemical analysis showed the presence of TGF-β1 (47% of tumors), TGF-β2 (42% of tumors), and TGF-β3 (40% of tumors) in the cancer cells. The presence of TGF-β2 was associated with advanced tumor stage (P <0.05). Furthermore, there was a significant correlation between the absence of TGF-βs in the tumors and longer postoperative survival. Northern blot analysis indicated that, by comparison with the normal pancreas, pancreatic adenocarcinomas showed 11- (P <0.001), 7- (P <0.05), and 9-fold (P <0.001) increases in the messenger RNA (mRNA) levels encoding TGF-β1, TGF-β2, and TGF-β3, respectively. By in situ hybridization, these mRNA moieties colocalized with their respective proteins in the cancer cells. Conclusions: These findings show that human pancreatic cancers show increased levels of TGF-β isoforms and enhanced TGF-β mRNA expression and suggest that the presence of TGF-βs in pancreatic cancer cells may contribute to disease progression.

Original languageEnglish (US)
Pages (from-to)1846-1856
Number of pages11
JournalGastroenterology
Volume105
Issue number6
StatePublished - 1993
Externally publishedYes

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Transforming Growth Factors
Pancreatic Neoplasms
Protein Isoforms
Survival
Neoplasms
Northern Blotting
Messenger RNA
In Situ Hybridization
Disease Progression
Pancreas
Intercellular Signaling Peptides and Proteins
Adenocarcinoma
Cell Proliferation
Peptides
Growth
Proteins

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Friess, H., Yamanaka, Y., Büchler, M., Ebert, M., Beger, H. G., Gold, L. I., & Korc, M. (1993). Enhanced expression of transforming growth factor β isoforms in pancreatic cancer correlates with decreased survival. Gastroenterology, 105(6), 1846-1856.

Enhanced expression of transforming growth factor β isoforms in pancreatic cancer correlates with decreased survival. / Friess, Helmut; Yamanaka, Yoichiro; Büchler, Markus; Ebert, Matthias; Beger, Hans G.; Gold, Leslie I.; Korc, Murray.

In: Gastroenterology, Vol. 105, No. 6, 1993, p. 1846-1856.

Research output: Contribution to journalArticle

Friess, H, Yamanaka, Y, Büchler, M, Ebert, M, Beger, HG, Gold, LI & Korc, M 1993, 'Enhanced expression of transforming growth factor β isoforms in pancreatic cancer correlates with decreased survival', Gastroenterology, vol. 105, no. 6, pp. 1846-1856.
Friess H, Yamanaka Y, Büchler M, Ebert M, Beger HG, Gold LI et al. Enhanced expression of transforming growth factor β isoforms in pancreatic cancer correlates with decreased survival. Gastroenterology. 1993;105(6):1846-1856.
Friess, Helmut ; Yamanaka, Yoichiro ; Büchler, Markus ; Ebert, Matthias ; Beger, Hans G. ; Gold, Leslie I. ; Korc, Murray. / Enhanced expression of transforming growth factor β isoforms in pancreatic cancer correlates with decreased survival. In: Gastroenterology. 1993 ; Vol. 105, No. 6. pp. 1846-1856.
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AU - Yamanaka, Yoichiro

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AU - Gold, Leslie I.

AU - Korc, Murray

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