Enhanced hematopoietic activity of a human granulocyte/macrophage colony-stimulating factor-interleukin 3 fusion protein

Benson M. Curtis, Douglas E. Williams, Hal E. Broxmeyer, Joseph Dunn, Theresa Farrah, Eric Jeffery, William Clevenger, Paul Deroos, Unja Martin, Della Friend, Vickie Craig, Richard Gayle, Virginia Price, David Cosman, Carl J. March, Linda S. Park

Research output: Contribution to journalArticle

123 Scopus citations

Abstract

Granulocyte/macrophage colony-stimulating factor-interleukin 3 (GM-CSF-IL-3) fusion proteins were generated by construction of a plasmid in which the coding regions of human GM-CSF and IL-3 cDNAs were connected by a synthetic linker sequence followed by subsequent expression in yeast. Both GM-CSF-IL-3 and IL-3-GM-CSF fusion proteins were purified to homogeneity and shown to bind to cell-surface receptors through either their GM-CSF or IL-3 domains. The fusion proteins exhibited enhanced receptor affinity, proliferative activity, and hematopoietic colony-stimulating activity compared with either IL-3 and/or GM-CSF alone. This suggests that GM-CSF-IL-3 fusion proteins may hold future promise as therapeutic agents.

Original languageEnglish (US)
Pages (from-to)5809-5813
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number13
DOIs
StatePublished - Jul 1 1991

Keywords

  • Growth factors
  • Hematopoiesis

ASJC Scopus subject areas

  • General
  • Genetics

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    Curtis, B. M., Williams, D. E., Broxmeyer, H. E., Dunn, J., Farrah, T., Jeffery, E., Clevenger, W., Deroos, P., Martin, U., Friend, D., Craig, V., Gayle, R., Price, V., Cosman, D., March, C. J., & Park, L. S. (1991). Enhanced hematopoietic activity of a human granulocyte/macrophage colony-stimulating factor-interleukin 3 fusion protein. Proceedings of the National Academy of Sciences of the United States of America, 88(13), 5809-5813. https://doi.org/10.1073/pnas.88.13.5809