Enhanced L-type Ca2+ channel current density in coronary smooth muscle of exercise-trained pigs is compensated to limit myoplasmic free Ca2+ accumulation

Cristine L. Heaps, Douglas K. Bowles, Michael Sturek, M. Harold Laughlin, Janet L. Parker

Research output: Contribution to journalArticle

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Abstract

1. We hypothesized that enhanced voltage-gated Ca2+ channel current (VGCC) density in coronary smooth muscle cells of exercise-trained miniature Yucatan pigs is compensated by other cellular Ca2+ regulatory mechanisms to limit net myoplasmic free Ca2+ accumulation. 2. Whole-cell voltage clamp experiments demonstrated enhanced VGCC density in smooth muscle cells freshly dispersed from coronary arteries of exercise-trained vs. sedentary animals. 3. In separate experiments using fura-2 microfluorometry, we measured depolarization-induced (80 mM KCl) accumulation of myoplasmic free Ba2+ and free Ca2+. Both maximal rate and net accumulation of free Ba2+ in response to membrane depolarization were increased in smooth muscle cells isolated from exercise-trained pigs, consistent with an increased VGCC density. Depolarization also produced an enhanced maximal rate of free Ca2+ accumulation in cells of exercise-trained pigs; however, net accumulation of free Ca2+ was not significantly increased suggesting enhanced Ca2+ influx was compensated to limit net free Ca2+ accumulation. 4. Inhibition of sarco-endoplasmic reticulum Ca2+-transporting ATPase (SERCA; 10 μM cyclopiazonic acid) and/or sarcolemmal Na+-Ca2+ exchange (low extracellular Na+) suggested neither mechanism compensated the enhanced VGCC in cells of exercise-trained animals. 5. Local Ca2+-dependent inactivation of VGCC, assessed by buffering myoplasmic Ca2+ with EGTA in the pipette and using Ca2+ and Ba2+ as charge carriers, was not different between cells of sedentary and exercise-trained animals. 6. Our findings indicate that increased VGCC density is compensated by other cellular Ca2+ regulatory mechanisms to limit net myoplasmic free Ca2+ accumulation in smooth muscle cells of exercise-trained animals. Further, SERCA, Na+-Ca2+ exchange and local Ca2+- dependent inactivation of VGCC do not appear to function as compensatory mechanisms. Additional potential compensatory mechanisms include Ca2+ extrusion via plasma membrane Ca2+-ATPase, mitochondrial uptake, myoplasmic Ca2+-binding proteins and other sources of VGCC inactivation.

Original languageEnglish (US)
Pages (from-to)435-445
Number of pages11
JournalJournal of Physiology
Volume528
Issue number3
StatePublished - Nov 1 2000
Externally publishedYes

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Smooth Muscle Myocytes
Smooth Muscle
Swine
Sarcoplasmic Reticulum Calcium-Transporting ATPases
Cytophotometry
Calcium-Transporting ATPases
Fura-2
Egtazic Acid
Coronary Vessels
Carrier Proteins
Cell Membrane
Membranes

ASJC Scopus subject areas

  • Physiology

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Enhanced L-type Ca2+ channel current density in coronary smooth muscle of exercise-trained pigs is compensated to limit myoplasmic free Ca2+ accumulation. / Heaps, Cristine L.; Bowles, Douglas K.; Sturek, Michael; Laughlin, M. Harold; Parker, Janet L.

In: Journal of Physiology, Vol. 528, No. 3, 01.11.2000, p. 435-445.

Research output: Contribution to journalArticle

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