Enhanced strength of endothelial attachment on polyester elastomer and polytetrafluoroethylene graft surfaces with fibronectin substrate

Kenneth Kesler, Malcolm B. Herring, Michael P. Arnold, John L. Glover, Hee Myung Park, Michael N. Helmus, Phillip J. Bendick

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Successful development of a vascular prosthesis lined with endothelium may depend on the ability of the attached cells to resist shear forces after implantation. The purpose of this article is to describe a model for measurement of endothelial detachment caused by shear stress and to identify biomaterials that resist loss of attached cells as a result of shear stress. With human umbilical venous endothelium labeled with indium 111-oxine, cellular attachment to uncoated and fibronectin-coated polyester elastomer and expanded polytetrafluoroethylene (e-PTFE) graft surfaces was quantified after an 18-hour incubation PTFE grafts prepared by immediate seeding were also studied. The relative strength of endothelial attachment was determined by the percentage of the original inoculum remaining after the seeded graft surfaces were subjected to a physiologic shear stress of 15 dynes/cm2 during in vitro perfusion. In polyester elastomer grafts, fibronectin did not significantly increase initial attachment but did increase the percentage of inoculum remaining after perfusion (92.1% vs. 39.7%, p = 0.001). A similar relationship existed between fibronectin-coated e-PTFE and immediately seeded e-PTFE preparations with 61.6% and 25.8%, respectively, of the inoculum remaining after perfusion (p = 0.001). Furthermore, the percentage of inoculum retained on fibronectin-coated polyester elastomer was significantly greater than on fibronectin-coated e-PTFE (p = 0.001). In comparing uncoated grafts, polyester elastomer had 39.7% of the inoculum retained after perfusion whereas only 1.8% was remaining on the e-PTFE grafts (p = 0.0001). We conclude that polyester elastomer permits better endothelial cell attachment than e-PTFE and that fibronectin coating enhances the strength of attachment to both graft materials.

Original languageEnglish
Pages (from-to)58-64
Number of pages7
JournalJournal of Vascular Surgery
Volume3
Issue number1
DOIs
StatePublished - 1986

Fingerprint

Elastomers
Polyesters
Polytetrafluoroethylene
Fibronectins
Transplants
Perfusion
Endothelium
Blood Vessel Prosthesis
Umbilicus
Biocompatible Materials
Endothelial Cells

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Enhanced strength of endothelial attachment on polyester elastomer and polytetrafluoroethylene graft surfaces with fibronectin substrate. / Kesler, Kenneth; Herring, Malcolm B.; Arnold, Michael P.; Glover, John L.; Park, Hee Myung; Helmus, Michael N.; Bendick, Phillip J.

In: Journal of Vascular Surgery, Vol. 3, No. 1, 1986, p. 58-64.

Research output: Contribution to journalArticle

Kesler, Kenneth ; Herring, Malcolm B. ; Arnold, Michael P. ; Glover, John L. ; Park, Hee Myung ; Helmus, Michael N. ; Bendick, Phillip J. / Enhanced strength of endothelial attachment on polyester elastomer and polytetrafluoroethylene graft surfaces with fibronectin substrate. In: Journal of Vascular Surgery. 1986 ; Vol. 3, No. 1. pp. 58-64.
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