Enhanced synaptic connectivity and epilepsy in C1q knockout mice

Yunxiang Chu, Xiaoming Jin, Isabel Parada, Alexei Pesic, Beth Stevens, Ben Barres, David A. Prince

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Excessive CNS synapses are eliminated during development to establish mature patterns of neuronal connectivity. A complement cascade protein, C1q, is involved in this process.Mice deficient in C1q fail to refine retinogeniculate connections resulting in excessive retinal innervation of lateral geniculate neurons. We hypothesized that C1q knockout (KO) mice would exhibit defects in neocortical synapse elimination resulting in enhanced excitatory synaptic connectivity and epileptiform activity. We recorded spontaneous and evoked field potential activity in neocortical slices and obtained video-EEG recordings from implanted C1q KO and wild-type (WT) mice. We also used laser scanning photostimulation of caged glutamate and whole cell recordings to map excitatory and inhibitory synaptic connectivity. Spontaneous and evoked epileptiform field potentials occurred at multiple sites inneocortical slices from C1q KO, but not WT mice. Laser mapping experiments in C1q KO slices showed that the proportion of glutamate uncaging sites from which excitatory postsynaptic currents (EPSCs) could be evoked ("hotspot ratio") increased significantly in layer IV and layer V, although EPSC amplitudes were unaltered. Density of axonal boutons was significantly increased in layer V pyramidal neurons of C1q KO mice. Implanted KO mice had frequent behavioral seizures consisting of behavioral arrest associated with bihemispheric spikes and slow wave activity lasting from 5 to 30 s. Results indicate that epileptogenesis in C1q KO mice is related to a genetically determined failure to prune excessive excitatory synapses during development.

Original languageEnglish
Pages (from-to)7975-7980
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number17
DOIs
StatePublished - Apr 27 2010

Fingerprint

Knockout Mice
Epilepsy
Synapses
Excitatory Postsynaptic Potentials
Glutamic Acid
Lasers
Video Recording
Pyramidal Cells
Patch-Clamp Techniques
Evoked Potentials
Electroencephalography
Complement System Proteins
Seizures
Neurons

Keywords

  • Axonal boutons
  • Complement cascade
  • Development
  • Seizure
  • Synaptic pruning

ASJC Scopus subject areas

  • General

Cite this

Enhanced synaptic connectivity and epilepsy in C1q knockout mice. / Chu, Yunxiang; Jin, Xiaoming; Parada, Isabel; Pesic, Alexei; Stevens, Beth; Barres, Ben; Prince, David A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 17, 27.04.2010, p. 7975-7980.

Research output: Contribution to journalArticle

Chu, Yunxiang ; Jin, Xiaoming ; Parada, Isabel ; Pesic, Alexei ; Stevens, Beth ; Barres, Ben ; Prince, David A. / Enhanced synaptic connectivity and epilepsy in C1q knockout mice. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 17. pp. 7975-7980.
@article{3f474e5accb041a8a672f7adf299379e,
title = "Enhanced synaptic connectivity and epilepsy in C1q knockout mice",
abstract = "Excessive CNS synapses are eliminated during development to establish mature patterns of neuronal connectivity. A complement cascade protein, C1q, is involved in this process.Mice deficient in C1q fail to refine retinogeniculate connections resulting in excessive retinal innervation of lateral geniculate neurons. We hypothesized that C1q knockout (KO) mice would exhibit defects in neocortical synapse elimination resulting in enhanced excitatory synaptic connectivity and epileptiform activity. We recorded spontaneous and evoked field potential activity in neocortical slices and obtained video-EEG recordings from implanted C1q KO and wild-type (WT) mice. We also used laser scanning photostimulation of caged glutamate and whole cell recordings to map excitatory and inhibitory synaptic connectivity. Spontaneous and evoked epileptiform field potentials occurred at multiple sites inneocortical slices from C1q KO, but not WT mice. Laser mapping experiments in C1q KO slices showed that the proportion of glutamate uncaging sites from which excitatory postsynaptic currents (EPSCs) could be evoked ({"}hotspot ratio{"}) increased significantly in layer IV and layer V, although EPSC amplitudes were unaltered. Density of axonal boutons was significantly increased in layer V pyramidal neurons of C1q KO mice. Implanted KO mice had frequent behavioral seizures consisting of behavioral arrest associated with bihemispheric spikes and slow wave activity lasting from 5 to 30 s. Results indicate that epileptogenesis in C1q KO mice is related to a genetically determined failure to prune excessive excitatory synapses during development.",
keywords = "Axonal boutons, Complement cascade, Development, Seizure, Synaptic pruning",
author = "Yunxiang Chu and Xiaoming Jin and Isabel Parada and Alexei Pesic and Beth Stevens and Ben Barres and Prince, {David A.}",
year = "2010",
month = "4",
day = "27",
doi = "10.1073/pnas.0913449107",
language = "English",
volume = "107",
pages = "7975--7980",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "17",

}

TY - JOUR

T1 - Enhanced synaptic connectivity and epilepsy in C1q knockout mice

AU - Chu, Yunxiang

AU - Jin, Xiaoming

AU - Parada, Isabel

AU - Pesic, Alexei

AU - Stevens, Beth

AU - Barres, Ben

AU - Prince, David A.

PY - 2010/4/27

Y1 - 2010/4/27

N2 - Excessive CNS synapses are eliminated during development to establish mature patterns of neuronal connectivity. A complement cascade protein, C1q, is involved in this process.Mice deficient in C1q fail to refine retinogeniculate connections resulting in excessive retinal innervation of lateral geniculate neurons. We hypothesized that C1q knockout (KO) mice would exhibit defects in neocortical synapse elimination resulting in enhanced excitatory synaptic connectivity and epileptiform activity. We recorded spontaneous and evoked field potential activity in neocortical slices and obtained video-EEG recordings from implanted C1q KO and wild-type (WT) mice. We also used laser scanning photostimulation of caged glutamate and whole cell recordings to map excitatory and inhibitory synaptic connectivity. Spontaneous and evoked epileptiform field potentials occurred at multiple sites inneocortical slices from C1q KO, but not WT mice. Laser mapping experiments in C1q KO slices showed that the proportion of glutamate uncaging sites from which excitatory postsynaptic currents (EPSCs) could be evoked ("hotspot ratio") increased significantly in layer IV and layer V, although EPSC amplitudes were unaltered. Density of axonal boutons was significantly increased in layer V pyramidal neurons of C1q KO mice. Implanted KO mice had frequent behavioral seizures consisting of behavioral arrest associated with bihemispheric spikes and slow wave activity lasting from 5 to 30 s. Results indicate that epileptogenesis in C1q KO mice is related to a genetically determined failure to prune excessive excitatory synapses during development.

AB - Excessive CNS synapses are eliminated during development to establish mature patterns of neuronal connectivity. A complement cascade protein, C1q, is involved in this process.Mice deficient in C1q fail to refine retinogeniculate connections resulting in excessive retinal innervation of lateral geniculate neurons. We hypothesized that C1q knockout (KO) mice would exhibit defects in neocortical synapse elimination resulting in enhanced excitatory synaptic connectivity and epileptiform activity. We recorded spontaneous and evoked field potential activity in neocortical slices and obtained video-EEG recordings from implanted C1q KO and wild-type (WT) mice. We also used laser scanning photostimulation of caged glutamate and whole cell recordings to map excitatory and inhibitory synaptic connectivity. Spontaneous and evoked epileptiform field potentials occurred at multiple sites inneocortical slices from C1q KO, but not WT mice. Laser mapping experiments in C1q KO slices showed that the proportion of glutamate uncaging sites from which excitatory postsynaptic currents (EPSCs) could be evoked ("hotspot ratio") increased significantly in layer IV and layer V, although EPSC amplitudes were unaltered. Density of axonal boutons was significantly increased in layer V pyramidal neurons of C1q KO mice. Implanted KO mice had frequent behavioral seizures consisting of behavioral arrest associated with bihemispheric spikes and slow wave activity lasting from 5 to 30 s. Results indicate that epileptogenesis in C1q KO mice is related to a genetically determined failure to prune excessive excitatory synapses during development.

KW - Axonal boutons

KW - Complement cascade

KW - Development

KW - Seizure

KW - Synaptic pruning

UR - http://www.scopus.com/inward/record.url?scp=77952403414&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952403414&partnerID=8YFLogxK

U2 - 10.1073/pnas.0913449107

DO - 10.1073/pnas.0913449107

M3 - Article

C2 - 20375278

AN - SCOPUS:77952403414

VL - 107

SP - 7975

EP - 7980

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 17

ER -