Enhanced TLR-MYD88 signaling stimulates autoinflammation in SH3BP2 cherubism mice and defines the etiology of cherubism

Teruhito Yoshitaka, Tomoyuki Mukai, Mizuho Kittaka, Lisa M. Alford, Salome Masrani, Shu Ishida, Ken Yamaguchi, Motohiko Yamada, Noriyoshi Mizuno, Bjorn R. Olsen, Ernst J. Reichenberger, Yasuyoshi Ueki

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Cherubism is caused by mutations in SH3BP2. Studies of cherubism mice showed that tumor necrosis factor α (TNF-α)-dependent autoinflammation isamajor cause of the disorder but failed to explain why human cherubism lesions are restricted to jaws andregress after puberty. We demonstrate that the inflammation in cherubism mice is MYD88 dependent and is rescued in the absence of TLR2 and TLR4. However, germ-free cherubism mice also develop inflammation. Mutant macrophages are hyperresponsive to PAMPs (pathogen-associated molecular patterns) and DAMPs (damage-associated molecular patterns) that activate Toll-like receptors (TLRs), resulting in TNF-α overproduction. Phosphorylation of SH3BP2 at Y183 is critical for theTNF-α production. Finally, SYK depletion in macrophages prevents the inflammation. These data suggest that the presence of a large amount of TLR ligands, presumably oral bacteria and DAMPs duringjawbone remodeling, may cause the jaw-specific development of human cherubism lesions. Reduced levels of DAMPs after stabilization of jaw remodeling may contribute to the age-dependent regression.

Original languageEnglish (US)
Pages (from-to)1752-1766
Number of pages15
JournalCell Reports
Volume8
Issue number6
DOIs
StatePublished - Sep 25 2014
Externally publishedYes

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Cherubism
Macrophages
Toll-Like Receptors
Tumor Necrosis Factor-alpha
Phosphorylation
Jaw
Bacteria
Stabilization
Ligands
Inflammation
Human Development
Puberty
Mutation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Enhanced TLR-MYD88 signaling stimulates autoinflammation in SH3BP2 cherubism mice and defines the etiology of cherubism. / Yoshitaka, Teruhito; Mukai, Tomoyuki; Kittaka, Mizuho; Alford, Lisa M.; Masrani, Salome; Ishida, Shu; Yamaguchi, Ken; Yamada, Motohiko; Mizuno, Noriyoshi; Olsen, Bjorn R.; Reichenberger, Ernst J.; Ueki, Yasuyoshi.

In: Cell Reports, Vol. 8, No. 6, 25.09.2014, p. 1752-1766.

Research output: Contribution to journalArticle

Yoshitaka, T, Mukai, T, Kittaka, M, Alford, LM, Masrani, S, Ishida, S, Yamaguchi, K, Yamada, M, Mizuno, N, Olsen, BR, Reichenberger, EJ & Ueki, Y 2014, 'Enhanced TLR-MYD88 signaling stimulates autoinflammation in SH3BP2 cherubism mice and defines the etiology of cherubism', Cell Reports, vol. 8, no. 6, pp. 1752-1766. https://doi.org/10.1016/j.celrep.2014.08.023
Yoshitaka, Teruhito ; Mukai, Tomoyuki ; Kittaka, Mizuho ; Alford, Lisa M. ; Masrani, Salome ; Ishida, Shu ; Yamaguchi, Ken ; Yamada, Motohiko ; Mizuno, Noriyoshi ; Olsen, Bjorn R. ; Reichenberger, Ernst J. ; Ueki, Yasuyoshi. / Enhanced TLR-MYD88 signaling stimulates autoinflammation in SH3BP2 cherubism mice and defines the etiology of cherubism. In: Cell Reports. 2014 ; Vol. 8, No. 6. pp. 1752-1766.
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