Enhancing excitatory activity of somatosensory cortex alleviates neuropathic pain through regulating homeostatic plasticity

Wenhui Xiong, Xingjie Ping, Matthew S. Ripsch, Grace Santa Cruz Chavez, Heidi Elise Hannon, Kewen Jiang, Chunhui Bao, Vaishnavi Jadhav, Lifang Chen, Zhi Chai, Cungen Ma, Huangan Wu, Jianqiao Feng, Armin Blesch, Fletcher White, Xiaoming Jin

Research output: Contribution to journalArticle

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Abstract

Central sensitization and network hyperexcitability of the nociceptive system is a basic mechanism of neuropathic pain. We hypothesize that development of cortical hyperexcitability underlying neuropathic pain may involve homeostatic plasticity in response to lesion-induced somatosensory deprivation and activity loss, and can be controlled by enhancing cortical activity. In a mouse model of neuropathic pain, in vivo two-photon imaging and patch clamp recording showed initial loss and subsequent recovery and enhancement of spontaneous firings of somatosensory cortical pyramidal neurons. Unilateral optogenetic stimulation of cortical pyramidal neurons both prevented and reduced pain-like behavior as detected by bilateral mechanical hypersensitivity of hindlimbs, but corpus callosotomy eliminated the analgesic effect that was ipsilateral, but not contralateral, to optogenetic stimulation, suggesting involvement of inter-hemispheric excitatory drive in this effect. Enhancing activity by focally blocking cortical GABAergic inhibition had a similar relieving effect on the pain-like behavior. Patch clamp recordings from layer V pyramidal neurons showed that optogenetic stimulation normalized cortical hyperexcitability through changing neuronal membrane properties and reducing frequency of excitatory postsynaptic events. We conclude that development of neuropathic pain involves abnormal homeostatic activity regulation of somatosensory cortex, and that enhancing cortical excitatory activity may be a novel strategy for preventing and controlling neuropathic pain.

Original languageEnglish (US)
Article number12743
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Somatosensory Cortex
Neuralgia
Optogenetics
Pyramidal Cells
Central Nervous System Sensitization
Pain
Hindlimb
Photons
Analgesics
Hypersensitivity
Membranes

ASJC Scopus subject areas

  • General

Cite this

Enhancing excitatory activity of somatosensory cortex alleviates neuropathic pain through regulating homeostatic plasticity. / Xiong, Wenhui; Ping, Xingjie; Ripsch, Matthew S.; Chavez, Grace Santa Cruz; Elise Hannon, Heidi; Jiang, Kewen; Bao, Chunhui; Jadhav, Vaishnavi; Chen, Lifang; Chai, Zhi; Ma, Cungen; Wu, Huangan; Feng, Jianqiao; Blesch, Armin; White, Fletcher; Jin, Xiaoming.

In: Scientific Reports, Vol. 7, No. 1, 12743, 01.12.2017.

Research output: Contribution to journalArticle

Xiong, W, Ping, X, Ripsch, MS, Chavez, GSC, Elise Hannon, H, Jiang, K, Bao, C, Jadhav, V, Chen, L, Chai, Z, Ma, C, Wu, H, Feng, J, Blesch, A, White, F & Jin, X 2017, 'Enhancing excitatory activity of somatosensory cortex alleviates neuropathic pain through regulating homeostatic plasticity', Scientific Reports, vol. 7, no. 1, 12743. https://doi.org/10.1038/s41598-017-12972-6
Xiong, Wenhui ; Ping, Xingjie ; Ripsch, Matthew S. ; Chavez, Grace Santa Cruz ; Elise Hannon, Heidi ; Jiang, Kewen ; Bao, Chunhui ; Jadhav, Vaishnavi ; Chen, Lifang ; Chai, Zhi ; Ma, Cungen ; Wu, Huangan ; Feng, Jianqiao ; Blesch, Armin ; White, Fletcher ; Jin, Xiaoming. / Enhancing excitatory activity of somatosensory cortex alleviates neuropathic pain through regulating homeostatic plasticity. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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