Enterocyte-like differentiation of the Caco-2 intestinal cell line is associated with increases in AP-1 protein binding

Qingming Ding, Zizheng Dong, B. Mark Evers

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The nuclear factor AP-1, a large family of transcription factors composed of the Jun and Fos protein families, plays a role in the differentiation of various cells; the role of the AP, 1 factors in intestinal differentiation is not known. Members of the AP-1 family can be activated by the Ras pathway and, in addition, Ras appears to be important for gut differentiation. The purpose of this study was to determine whether AP-1 activity is altered in the Caco-2 cell lines which spontaneously differentiates to a small bowel phenotype after confluency, and the Caco-2- ras cell line, which exhibits differentiated properties regardless of culture conditions. AP-1 binding activity, consisting of c-Jun, JunD, c-Fos and Fra- 2 proteins, was increased in Caco-2 cells at 3 days postconfluency, a time point associated with G1 block and cessation of proliferation. Steady state levels of JunD were increased at day 3 postconfluency as determined by Western blot. Furthermore, AP-1 binding was increased in preconfluent Caco- 2-ras cells compared with parental Caco-2 cells, suggesting that AP-1 induction may be mediated by the Ras pathway. The early induction of AP-1 binding activity suggests a role for these proteins in the differentiation of the Caco-2 intestinal cell line.

Original languageEnglish (US)
Pages (from-to)175-182
Number of pages8
JournalLife Sciences
Volume64
Issue number3
DOIs
StatePublished - Dec 11 1998
Externally publishedYes

Fingerprint

Caco-2 Cells
Enterocytes
Transcription Factor AP-1
Protein Binding
Cells
Cell Line
NFI Transcription Factors
Proteins
Cell Differentiation
Transcription Factors
Western Blotting
Phenotype

Keywords

  • AP-1 factors
  • Caco-2 cell line
  • Intestinal differentiation

ASJC Scopus subject areas

  • Pharmacology

Cite this

Enterocyte-like differentiation of the Caco-2 intestinal cell line is associated with increases in AP-1 protein binding. / Ding, Qingming; Dong, Zizheng; Evers, B. Mark.

In: Life Sciences, Vol. 64, No. 3, 11.12.1998, p. 175-182.

Research output: Contribution to journalArticle

Ding, Qingming ; Dong, Zizheng ; Evers, B. Mark. / Enterocyte-like differentiation of the Caco-2 intestinal cell line is associated with increases in AP-1 protein binding. In: Life Sciences. 1998 ; Vol. 64, No. 3. pp. 175-182.
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