A spectrophotometric endpoint assay for determination of branched-chain α-keto acids is described. The assay depends on measurement of the NADH produced after addition of branched-chain α-keto acid dehydrogenase. Interference by pyruvate and α-ketobutyrate was eliminated by pretreating the sample with pyruvate dehydrogenase. The method yielded a peripheral venous plasma value of 59 ± 5 μm (mean ± SE) for the branched-chain α-keto acids of five overnight fasted healthy humans.
- branched-chain amino acids
- branched-chain α-keto acid dehydrogenase
- branched-chain α-keto acids
ASJC Scopus subject areas
- Molecular Biology