Enzymatic determination of the branched-chain α-keto acids

G. W. Goodwin, M. J. Kuntz, R. Paxton, Robert Harris

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A spectrophotometric endpoint assay for determination of branched-chain α-keto acids is described. The assay depends on measurement of the NADH produced after addition of branched-chain α-keto acid dehydrogenase. Interference by pyruvate and α-ketobutyrate was eliminated by pretreating the sample with pyruvate dehydrogenase. The method yielded a peripheral venous plasma value of 59 ± 5 μm (mean ± SE) for the branched-chain α-keto acids of five overnight fasted healthy humans.

Original languageEnglish
Pages (from-to)536-539
Number of pages4
JournalAnalytical Biochemistry
Volume162
Issue number2
DOIs
StatePublished - May 1 1987

Fingerprint

Endpoint Determination
Keto Acids
Pyruvic Acid
Assays
3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
NAD
Oxidoreductases
Plasmas

Keywords

  • blood
  • branched-chain amino acids
  • branched-chain α-keto acid dehydrogenase
  • branched-chain α-keto acids
  • leucine
  • plasma

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Enzymatic determination of the branched-chain α-keto acids. / Goodwin, G. W.; Kuntz, M. J.; Paxton, R.; Harris, Robert.

In: Analytical Biochemistry, Vol. 162, No. 2, 01.05.1987, p. 536-539.

Research output: Contribution to journalArticle

Goodwin, G. W. ; Kuntz, M. J. ; Paxton, R. ; Harris, Robert. / Enzymatic determination of the branched-chain α-keto acids. In: Analytical Biochemistry. 1987 ; Vol. 162, No. 2. pp. 536-539.
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