Enzymatic determination of the branched-chain α-keto acids

G. W. Goodwin, M. J. Kuntz, R. Paxton, R. A. Harris

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

A spectrophotometric endpoint assay for determination of branched-chain α-keto acids is described. The assay depends on measurement of the NADH produced after addition of branched-chain α-keto acid dehydrogenase. Interference by pyruvate and α-ketobutyrate was eliminated by pretreating the sample with pyruvate dehydrogenase. The method yielded a peripheral venous plasma value of 59 ± 5 μm (mean ± SE) for the branched-chain α-keto acids of five overnight fasted healthy humans.

Original languageEnglish (US)
Pages (from-to)536-539
Number of pages4
JournalAnalytical biochemistry
Volume162
Issue number2
DOIs
StatePublished - May 1 1987

Keywords

  • blood
  • branched-chain amino acids
  • branched-chain α-keto acid dehydrogenase
  • branched-chain α-keto acids
  • leucine
  • plasma

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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