Eosinophil chemotactic factor-L (ECF-L) enhances osteoclast formation by increasing ICAM-1 expression

Veronica Garcia Palacios, Ho Yeon Chung, Sun Jin Choi, Noriyoshi Kurihara, Jun Won Lee, Lori A. Ehrlich, Robert Collins, G. David Roodman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Eosinophil chemotactic factor-L (ECF-L) is a novel stimulator of osteoclast (OCL) formation that acts at the differentiation/fusion stage of OCL formation, and is a cofactor for RANK ligand (RANKL). We examined the effects of ECF-L on the intracellular signaling pathways utilized by RANKL, and on the expression of ICAM-1/LFA-1 to determine its mechanism of action. RAW 264.7 and bone marrow cells were treated with RANKL and/or ECF-L Fc protein to determine their effect on NF-κB and AP-1 activity. ECF-L by itself only modestly increased NF-κB binding and JNK activity in RAW 264.7 cells, which were further enhanced by RANKL. In contrast, ECF-L Fc increased LFA-1α and ICAM-1 mRNA levels 1.8-fold in mouse marrow cultures, and anti-ICAM-1 almost completely inhibited OCL formation induced by 10-10 M 1,25-(OH) 2D3, and ECF-L Fc. Furthermore, ECF-L Fc did not enhance OCL formation by ICAM-1 knockout (KO) cells. Increased expression of ICAM-1 by ECF-L appears to be critical for its effects on OCL formation.

Original languageEnglish (US)
Pages (from-to)240-243
Number of pages4
JournalAnnals of the New York Academy of Sciences
Volume1068
Issue number1
DOIs
StatePublished - Apr 2006
Externally publishedYes

Fingerprint

Eosinophil Chemotactic Factors
Osteoclasts
Intercellular Adhesion Molecule-1
RANK Ligand
Lymphocyte Function-Associated Antigen-1
Transcription Factor AP-1
Bone Marrow Cells
Bone
Fusion reactions
Bone Marrow
Cells

Keywords

  • ECF-L
  • ICAM-1
  • Osteoclasts

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Eosinophil chemotactic factor-L (ECF-L) enhances osteoclast formation by increasing ICAM-1 expression. / Palacios, Veronica Garcia; Chung, Ho Yeon; Choi, Sun Jin; Kurihara, Noriyoshi; Lee, Jun Won; Ehrlich, Lori A.; Collins, Robert; Roodman, G. David.

In: Annals of the New York Academy of Sciences, Vol. 1068, No. 1, 04.2006, p. 240-243.

Research output: Contribution to journalArticle

Palacios, Veronica Garcia ; Chung, Ho Yeon ; Choi, Sun Jin ; Kurihara, Noriyoshi ; Lee, Jun Won ; Ehrlich, Lori A. ; Collins, Robert ; Roodman, G. David. / Eosinophil chemotactic factor-L (ECF-L) enhances osteoclast formation by increasing ICAM-1 expression. In: Annals of the New York Academy of Sciences. 2006 ; Vol. 1068, No. 1. pp. 240-243.
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