Epidemiology of colonization by nontypable Haemophilus influenzae in children

A longitudinal study

Stanley Spinola, J. Peacock, F. W. Denny, D. L. Smith, J. G. Cannon

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Eighty-six nasopharyngeal isolates of Haemophilus influenzae were prospectively obtained from three children who attended a day care center from infancy until early childhood (five to seven years). A majority of the strains were nontypable. We analyzed strains by comparing their biotypes and by performing electrophoresis of outer membrane proteins on polyacrylamide gels. Profiles of outer membrane proteins were very heterogeneous and could not be used as the basis for the development of a subtyping scheme. The children characteristically carried a nasopharyngeal strain defined by a unique outer membrane pattern for a period of months, lost it, and then acquired a new strain. We probed the outer membrane proteins of a child's strains by the western blot technique with serum obtained serially from the child. Isolates whose outer membrane proteins appeared identical on strained gels generally had similar antigenic bands on western blots but were occasionally immunologically distinct. Serum immunoglobulins of the IgG class that reacted with the outer membrane proteins did not appear to change greatly over time or to play a role in preventing or terminating colonization. We conclude that nasopharyngeal colonization in children by nontypable H. influenzae is a dynamic process and that factors that cause loss and acquisition of strains remain to be determined.

Original languageEnglish (US)
Pages (from-to)100-109
Number of pages10
JournalJournal of Infectious Diseases
Volume154
Issue number1
StatePublished - 1986
Externally publishedYes

Fingerprint

Haemophilus influenzae
Longitudinal Studies
Membrane Proteins
Epidemiology
Western Blotting
Immunoglobulin Isotypes
Serum
Electrophoresis
Immunoglobulin G
Gels
Membranes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Epidemiology of colonization by nontypable Haemophilus influenzae in children : A longitudinal study. / Spinola, Stanley; Peacock, J.; Denny, F. W.; Smith, D. L.; Cannon, J. G.

In: Journal of Infectious Diseases, Vol. 154, No. 1, 1986, p. 100-109.

Research output: Contribution to journalArticle

Spinola, Stanley ; Peacock, J. ; Denny, F. W. ; Smith, D. L. ; Cannon, J. G. / Epidemiology of colonization by nontypable Haemophilus influenzae in children : A longitudinal study. In: Journal of Infectious Diseases. 1986 ; Vol. 154, No. 1. pp. 100-109.
@article{ba09f20ba3b8440783357839ca3a9651,
title = "Epidemiology of colonization by nontypable Haemophilus influenzae in children: A longitudinal study",
abstract = "Eighty-six nasopharyngeal isolates of Haemophilus influenzae were prospectively obtained from three children who attended a day care center from infancy until early childhood (five to seven years). A majority of the strains were nontypable. We analyzed strains by comparing their biotypes and by performing electrophoresis of outer membrane proteins on polyacrylamide gels. Profiles of outer membrane proteins were very heterogeneous and could not be used as the basis for the development of a subtyping scheme. The children characteristically carried a nasopharyngeal strain defined by a unique outer membrane pattern for a period of months, lost it, and then acquired a new strain. We probed the outer membrane proteins of a child's strains by the western blot technique with serum obtained serially from the child. Isolates whose outer membrane proteins appeared identical on strained gels generally had similar antigenic bands on western blots but were occasionally immunologically distinct. Serum immunoglobulins of the IgG class that reacted with the outer membrane proteins did not appear to change greatly over time or to play a role in preventing or terminating colonization. We conclude that nasopharyngeal colonization in children by nontypable H. influenzae is a dynamic process and that factors that cause loss and acquisition of strains remain to be determined.",
author = "Stanley Spinola and J. Peacock and Denny, {F. W.} and Smith, {D. L.} and Cannon, {J. G.}",
year = "1986",
language = "English (US)",
volume = "154",
pages = "100--109",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Epidemiology of colonization by nontypable Haemophilus influenzae in children

T2 - A longitudinal study

AU - Spinola, Stanley

AU - Peacock, J.

AU - Denny, F. W.

AU - Smith, D. L.

AU - Cannon, J. G.

PY - 1986

Y1 - 1986

N2 - Eighty-six nasopharyngeal isolates of Haemophilus influenzae were prospectively obtained from three children who attended a day care center from infancy until early childhood (five to seven years). A majority of the strains were nontypable. We analyzed strains by comparing their biotypes and by performing electrophoresis of outer membrane proteins on polyacrylamide gels. Profiles of outer membrane proteins were very heterogeneous and could not be used as the basis for the development of a subtyping scheme. The children characteristically carried a nasopharyngeal strain defined by a unique outer membrane pattern for a period of months, lost it, and then acquired a new strain. We probed the outer membrane proteins of a child's strains by the western blot technique with serum obtained serially from the child. Isolates whose outer membrane proteins appeared identical on strained gels generally had similar antigenic bands on western blots but were occasionally immunologically distinct. Serum immunoglobulins of the IgG class that reacted with the outer membrane proteins did not appear to change greatly over time or to play a role in preventing or terminating colonization. We conclude that nasopharyngeal colonization in children by nontypable H. influenzae is a dynamic process and that factors that cause loss and acquisition of strains remain to be determined.

AB - Eighty-six nasopharyngeal isolates of Haemophilus influenzae were prospectively obtained from three children who attended a day care center from infancy until early childhood (five to seven years). A majority of the strains were nontypable. We analyzed strains by comparing their biotypes and by performing electrophoresis of outer membrane proteins on polyacrylamide gels. Profiles of outer membrane proteins were very heterogeneous and could not be used as the basis for the development of a subtyping scheme. The children characteristically carried a nasopharyngeal strain defined by a unique outer membrane pattern for a period of months, lost it, and then acquired a new strain. We probed the outer membrane proteins of a child's strains by the western blot technique with serum obtained serially from the child. Isolates whose outer membrane proteins appeared identical on strained gels generally had similar antigenic bands on western blots but were occasionally immunologically distinct. Serum immunoglobulins of the IgG class that reacted with the outer membrane proteins did not appear to change greatly over time or to play a role in preventing or terminating colonization. We conclude that nasopharyngeal colonization in children by nontypable H. influenzae is a dynamic process and that factors that cause loss and acquisition of strains remain to be determined.

UR - http://www.scopus.com/inward/record.url?scp=0022462620&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022462620&partnerID=8YFLogxK

M3 - Article

VL - 154

SP - 100

EP - 109

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 1

ER -