Epidermal G1-chalone and transforming growth factor-β are two different endogenous inhibitors of epidermal cell proliferation

K. Hartmut Richter, Ruben Schnapke, Matthias Clauss, Gerhard Fürstenberger, Detlef Hinz, Friedrich Marks

Research output: Contribution to journalArticle

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Abstract

Epidermal G1-chalone and transforming growth factor-β (TGFβ), two endogenous inhibitors of epidermal cell proliferation, were compared with regard to several effects on epidermis in vivo and in vitro. Both factors inhibited DNA labeling in a rat tongue epithelial cell line, with similar kinetics and half-maximal effects at approximately 1 pg/ml (enriched chalone) and 1 ng/ml (TGFβ). For primary neonatal mouse keratinocytes, TGFβ was found to be a rather strong inhibitor of cell proliferation, whereas chalone showed only a weak effect on cells grown in medium containing 1.2 mM Ca2+ and no effect at all in the presence of 0.06 mM Ca2+. Vice versa, upon i.p. injection, only chalone was able to inhibit mouse epidermal DNA synthesis in vivo, whereas TGFβ had no effect at all. A moderate increase of transglutaminase activity in neonatal primary mouse keratinocytes was induced by both factors at concentrations of about 300 pg TGFβ/ml and 10 pg chalone fraction/ml. Chalone did not compete with [125I]TGFβ for specific binding sites on primary murine keratinocytes. A polyclonal "chalone antiserum" did not interact with TGFβ, and a neutralizing TGFβ antibody that inhibited the effect of TGFβ on cell proliferation could not block the inhibitory effect of chalone on RTE2 cells. In contrast to TGFβ, epidermal G1-chalone did not induce proliferation of NIH-3T3 cells. These results indicate that epidermal G1-chalone and TGFβ are two different inhibitors of epidermal cell proliferation.

Original languageEnglish (US)
Pages (from-to)496-504
Number of pages9
JournalJournal of Cellular Physiology
Volume142
Issue number3
StatePublished - Mar 1990
Externally publishedYes

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Cell proliferation
Transforming Growth Factors
Chalones
Cell Proliferation
Keratinocytes
G(1) chalone
Fibroblast Growth Factor 7
NIH 3T3 Cells
Transglutaminases
DNA
Tongue
Epidermis
Labeling
Rats
Immune Sera
Epithelial Cells
Binding Sites
Cells
Cell Line
Injections

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Epidermal G1-chalone and transforming growth factor-β are two different endogenous inhibitors of epidermal cell proliferation. / Richter, K. Hartmut; Schnapke, Ruben; Clauss, Matthias; Fürstenberger, Gerhard; Hinz, Detlef; Marks, Friedrich.

In: Journal of Cellular Physiology, Vol. 142, No. 3, 03.1990, p. 496-504.

Research output: Contribution to journalArticle

Richter, K. Hartmut ; Schnapke, Ruben ; Clauss, Matthias ; Fürstenberger, Gerhard ; Hinz, Detlef ; Marks, Friedrich. / Epidermal G1-chalone and transforming growth factor-β are two different endogenous inhibitors of epidermal cell proliferation. In: Journal of Cellular Physiology. 1990 ; Vol. 142, No. 3. pp. 496-504.
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