Epidermal growth factor binding is altered in pancreatic acini from diabetic rats

Murray Korc, Lynn M. Matrisian, Rieko Nakamura, Bruce E. Magun

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The effects of streptozotocin-induced diabetes on 125I-labeled epidermal growth factor (EGF) binding were studied in rat pancreatic acini. 125I-EGF binding was one-half maximal at 20 min, and maximal at 90 min. Saturation data revealed a decreased binding capacity in diabetic acini when compared with normal acini. Insulin, in vivo, normalized the decreased binding capacity. 125I-EGF internalization was also decreased in diabetic rat acini. Further, the inhibitory effect of cholecystokinin-octapeptide (CCK8) on cell-associated 125I-EGF radioactivity was significantly greater in diabetic than in normal rat acini. These findings suggest that insulin deficiency may lead to defective regulation of the exocrine pancreas by EGF.

Original languageEnglish (US)
Pages (from-to)2049-2055
Number of pages7
JournalLife Sciences
Volume35
Issue number20
DOIs
StatePublished - Nov 12 1984

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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