Epidermal growth factor receptor expression in pancreatic lesions induced in the rat by azaserine

C. J T Visser, R. A. De Weger, W. T M Van Blokland, I. Seifert-Bock, M. S. Kobrin, Murray Korc, R. A. Woutersen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In the present study, the expression of the epidermal growth factor receptor (EGFR) was investigated in putative preneoplastic and neoplastic acinar cell lesions induced in the rat pancreas by azaserine, using Northern blotting, in situ hybridisation (ISH) and immunohistochemistry. EGFR protein levels were decreased in putative preneoplastic eosinophilic acinar cell lesions (atypical acinar cell nodules, AACN) in comparison with normal acinar cells of the pancreas. However, EGFR mRNA expression correlated positively with the volume of AACN in pancreatic homogenates and ISH showed equal or stronger EGFR mRNA expression in AACN than in the surrounding normal acinar cells. Neither EGFR protein nor EGFR mRNA was detected in more advanced lesions such as acinar adenocarcinomas (in situ). Moreover, EGFR protein expression showed an inverse relationship with the mitotic rate of the acinar cells. These findings suggest that down-regulation of EGFR at the protein level may abrogate negative constraints on cell growth, which may stimulate the development of putative preneoplastic AACN to more advanced lesions and, ultimately, acinar adenocarcinomas.

Original languageEnglish (US)
Pages (from-to)92-98
Number of pages7
JournalBritish Journal of Cancer
Volume74
Issue number1
StatePublished - 1996
Externally publishedYes

Fingerprint

Azaserine
Acinar Cells
Epidermal Growth Factor Receptor
Messenger RNA
In Situ Hybridization
Pancreas
Proteins
Northern Blotting
Adenocarcinoma
Down-Regulation
Immunohistochemistry

Keywords

  • Acinar cell
  • Azaserine
  • Epidermal growth factor receptor
  • Pancreatic carcinogenesis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Visser, C. J. T., De Weger, R. A., Van Blokland, W. T. M., Seifert-Bock, I., Kobrin, M. S., Korc, M., & Woutersen, R. A. (1996). Epidermal growth factor receptor expression in pancreatic lesions induced in the rat by azaserine. British Journal of Cancer, 74(1), 92-98.

Epidermal growth factor receptor expression in pancreatic lesions induced in the rat by azaserine. / Visser, C. J T; De Weger, R. A.; Van Blokland, W. T M; Seifert-Bock, I.; Kobrin, M. S.; Korc, Murray; Woutersen, R. A.

In: British Journal of Cancer, Vol. 74, No. 1, 1996, p. 92-98.

Research output: Contribution to journalArticle

Visser, CJT, De Weger, RA, Van Blokland, WTM, Seifert-Bock, I, Kobrin, MS, Korc, M & Woutersen, RA 1996, 'Epidermal growth factor receptor expression in pancreatic lesions induced in the rat by azaserine', British Journal of Cancer, vol. 74, no. 1, pp. 92-98.
Visser CJT, De Weger RA, Van Blokland WTM, Seifert-Bock I, Kobrin MS, Korc M et al. Epidermal growth factor receptor expression in pancreatic lesions induced in the rat by azaserine. British Journal of Cancer. 1996;74(1):92-98.
Visser, C. J T ; De Weger, R. A. ; Van Blokland, W. T M ; Seifert-Bock, I. ; Kobrin, M. S. ; Korc, Murray ; Woutersen, R. A. / Epidermal growth factor receptor expression in pancreatic lesions induced in the rat by azaserine. In: British Journal of Cancer. 1996 ; Vol. 74, No. 1. pp. 92-98.
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