Epidermal growth factor receptor is required for colonic tumor promotion by dietary fat in the azoxymethane/dextran sulfate sodium model

Roles of transforming growth factor-α and PTGS2

Urszula Dougherty, Dario Cerasi, Ieva Taylor, Masha Kocherginsky, Ummuhan Tekin, Shamiram Badal, Lata Aluri, Amikar Sehdev, Sonia Cerda, Reba Mustafi, Jorge Delgado, Loren Joseph, Hongyan Zhu, John Hart, David Threadgill, Alessandro Fichera, Marc Bissonnette

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Purpose: Colon cancer is a major cause of cancer deaths. Dietary factors contribute substantially to the risk of this malignancy. Western-style diets promote development of azoxymethane-induced colon cancer. Although we showed that epidermal growth factor receptors (EGFR) controlled azoxymethane tumorigenesis in standard fat conditions, the role of EGFR in tumor promotion by high dietary fat has not been examined. Experimental Design: A/J x C57BL6/J mice with wild-type Egfr (Egfrwt) or loss-offunction waved-2 Egfr (Egfrwa2) received azoxymethane followed by standard (5% fat) or western-style (20% fat) diet. As F1 mice were resistant to azoxymethane, we treated mice with azoxymethane followed by one cycle of inflammation-inducing dextran sulfate sodium to induce tumorigenesis. Mice were sacrificed 12 weeks after dextran sulfate sodium. Tumors were graded for histology and assessed for EGFR ligands and proto-oncogenes by immunostaining, Western blotting, and realtime PCR. Results: Egfrwt mice gained significantly more weight and had exaggerated insulin resistance compared with Egfrwa2 mice on high-fat diet. Dietary fat promoted tumor incidence (71.2% versus 36.7%; P < 0.05) and cancer incidence (43.9% versus 16.7%; P < 0.05) only in Egfr wt mice. The lipid-rich diet also significantly increased tumor and cancer multiplicity only in Egfrwt mice. In tumors, dietary fat and Egfrwt upregulated transforming growth factor-α, amphiregulin, CTNNB1, MYC, and CCND1, whereas PTGS2 was only increased in Egfrwt mice and further upregulated by dietary fat. Notably, dietary fat increased transforming growth factor-á in normal colon. Conclusions: EGFR is required for dietary fat-induced weight gain and tumor promotion. EGFR-dependent increases in receptor ligands and PTGS2 likely drive diet-related tumor promotion.

Original languageEnglish (US)
Pages (from-to)6780-6789
Number of pages10
JournalClinical Cancer Research
Volume15
Issue number22
DOIs
StatePublished - Nov 15 2009
Externally publishedYes

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Azoxymethane
Dextran Sulfate
Dietary Fats
Transforming Growth Factors
Cyclooxygenase 2
Epidermal Growth Factor Receptor
Neoplasms
Diet
Fats
Colonic Neoplasms
Carcinogenesis
Ligands
Proto-Oncogenes
Incidence
High Fat Diet
Weight Gain
Insulin Resistance
Cause of Death
Histology
Colon

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Epidermal growth factor receptor is required for colonic tumor promotion by dietary fat in the azoxymethane/dextran sulfate sodium model : Roles of transforming growth factor-α and PTGS2. / Dougherty, Urszula; Cerasi, Dario; Taylor, Ieva; Kocherginsky, Masha; Tekin, Ummuhan; Badal, Shamiram; Aluri, Lata; Sehdev, Amikar; Cerda, Sonia; Mustafi, Reba; Delgado, Jorge; Joseph, Loren; Zhu, Hongyan; Hart, John; Threadgill, David; Fichera, Alessandro; Bissonnette, Marc.

In: Clinical Cancer Research, Vol. 15, No. 22, 15.11.2009, p. 6780-6789.

Research output: Contribution to journalArticle

Dougherty, U, Cerasi, D, Taylor, I, Kocherginsky, M, Tekin, U, Badal, S, Aluri, L, Sehdev, A, Cerda, S, Mustafi, R, Delgado, J, Joseph, L, Zhu, H, Hart, J, Threadgill, D, Fichera, A & Bissonnette, M 2009, 'Epidermal growth factor receptor is required for colonic tumor promotion by dietary fat in the azoxymethane/dextran sulfate sodium model: Roles of transforming growth factor-α and PTGS2', Clinical Cancer Research, vol. 15, no. 22, pp. 6780-6789. https://doi.org/10.1158/1078-0432.CCR-09-1678
Dougherty, Urszula ; Cerasi, Dario ; Taylor, Ieva ; Kocherginsky, Masha ; Tekin, Ummuhan ; Badal, Shamiram ; Aluri, Lata ; Sehdev, Amikar ; Cerda, Sonia ; Mustafi, Reba ; Delgado, Jorge ; Joseph, Loren ; Zhu, Hongyan ; Hart, John ; Threadgill, David ; Fichera, Alessandro ; Bissonnette, Marc. / Epidermal growth factor receptor is required for colonic tumor promotion by dietary fat in the azoxymethane/dextran sulfate sodium model : Roles of transforming growth factor-α and PTGS2. In: Clinical Cancer Research. 2009 ; Vol. 15, No. 22. pp. 6780-6789.
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abstract = "Purpose: Colon cancer is a major cause of cancer deaths. Dietary factors contribute substantially to the risk of this malignancy. Western-style diets promote development of azoxymethane-induced colon cancer. Although we showed that epidermal growth factor receptors (EGFR) controlled azoxymethane tumorigenesis in standard fat conditions, the role of EGFR in tumor promotion by high dietary fat has not been examined. Experimental Design: A/J x C57BL6/J mice with wild-type Egfr (Egfrwt) or loss-offunction waved-2 Egfr (Egfrwa2) received azoxymethane followed by standard (5{\%} fat) or western-style (20{\%} fat) diet. As F1 mice were resistant to azoxymethane, we treated mice with azoxymethane followed by one cycle of inflammation-inducing dextran sulfate sodium to induce tumorigenesis. Mice were sacrificed 12 weeks after dextran sulfate sodium. Tumors were graded for histology and assessed for EGFR ligands and proto-oncogenes by immunostaining, Western blotting, and realtime PCR. Results: Egfrwt mice gained significantly more weight and had exaggerated insulin resistance compared with Egfrwa2 mice on high-fat diet. Dietary fat promoted tumor incidence (71.2{\%} versus 36.7{\%}; P < 0.05) and cancer incidence (43.9{\%} versus 16.7{\%}; P < 0.05) only in Egfr wt mice. The lipid-rich diet also significantly increased tumor and cancer multiplicity only in Egfrwt mice. In tumors, dietary fat and Egfrwt upregulated transforming growth factor-α, amphiregulin, CTNNB1, MYC, and CCND1, whereas PTGS2 was only increased in Egfrwt mice and further upregulated by dietary fat. Notably, dietary fat increased transforming growth factor-{\'a} in normal colon. Conclusions: EGFR is required for dietary fat-induced weight gain and tumor promotion. EGFR-dependent increases in receptor ligands and PTGS2 likely drive diet-related tumor promotion.",
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T2 - Roles of transforming growth factor-α and PTGS2

AU - Dougherty, Urszula

AU - Cerasi, Dario

AU - Taylor, Ieva

AU - Kocherginsky, Masha

AU - Tekin, Ummuhan

AU - Badal, Shamiram

AU - Aluri, Lata

AU - Sehdev, Amikar

AU - Cerda, Sonia

AU - Mustafi, Reba

AU - Delgado, Jorge

AU - Joseph, Loren

AU - Zhu, Hongyan

AU - Hart, John

AU - Threadgill, David

AU - Fichera, Alessandro

AU - Bissonnette, Marc

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N2 - Purpose: Colon cancer is a major cause of cancer deaths. Dietary factors contribute substantially to the risk of this malignancy. Western-style diets promote development of azoxymethane-induced colon cancer. Although we showed that epidermal growth factor receptors (EGFR) controlled azoxymethane tumorigenesis in standard fat conditions, the role of EGFR in tumor promotion by high dietary fat has not been examined. Experimental Design: A/J x C57BL6/J mice with wild-type Egfr (Egfrwt) or loss-offunction waved-2 Egfr (Egfrwa2) received azoxymethane followed by standard (5% fat) or western-style (20% fat) diet. As F1 mice were resistant to azoxymethane, we treated mice with azoxymethane followed by one cycle of inflammation-inducing dextran sulfate sodium to induce tumorigenesis. Mice were sacrificed 12 weeks after dextran sulfate sodium. Tumors were graded for histology and assessed for EGFR ligands and proto-oncogenes by immunostaining, Western blotting, and realtime PCR. Results: Egfrwt mice gained significantly more weight and had exaggerated insulin resistance compared with Egfrwa2 mice on high-fat diet. Dietary fat promoted tumor incidence (71.2% versus 36.7%; P < 0.05) and cancer incidence (43.9% versus 16.7%; P < 0.05) only in Egfr wt mice. The lipid-rich diet also significantly increased tumor and cancer multiplicity only in Egfrwt mice. In tumors, dietary fat and Egfrwt upregulated transforming growth factor-α, amphiregulin, CTNNB1, MYC, and CCND1, whereas PTGS2 was only increased in Egfrwt mice and further upregulated by dietary fat. Notably, dietary fat increased transforming growth factor-á in normal colon. Conclusions: EGFR is required for dietary fat-induced weight gain and tumor promotion. EGFR-dependent increases in receptor ligands and PTGS2 likely drive diet-related tumor promotion.

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