Epidermal growth factor receptor restoration rescues the fatty liver regeneration in mice

Teresa Zimmers, Xiaoling Jin, Zongxiu Zhang, Yanlin Jiang, Leonidas Koniaris

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Hepatic steatosis is a common histological finding in obese patients. Even mild steatosis is associated with delayed hepatic regeneration and poor outcomes following liver resection or transplantation. We sought to identify and target molecular pathways that mediate this dysfunction. Lean mice and mice made obese through feeding of a high-fat, hypercaloric diet underwent 70 or 80% hepatectomy. After 70% resection, obese mice demonstrated 100% survival but experienced increased liver injury, reduced energy stores, reduced mitoses, increased necroapoptosis, and delayed recovery of liver mass. Increasing liver resection to 80% was associated with mortality of 40% in lean and 80% in obese mice (P < 0.05). Gene expression profiling showed decreased epidermal growth factor receptor (EGFR) in fatty liver. Meta-analysis of expression studies in mice, rats, and patients also demonstrated reduction of EGFR in fatty liver. In mice, both EGFR and phosphorylated EGFR decreased with increasing percent body fat. Hydrodynamic transfection of EGFR plasmids in mice corrected fatty liver regeneration, reducing liver injury, increasing proliferation, and improving survival after 80% resection. Loss of EGFR expression is rate limiting for liver regeneration in obesity. Therapies directed at increasing EGFR in steatosis might promote liver regeneration and survival following hepatic resection or transplantation.

Original languageEnglish (US)
Pages (from-to)E440-E449
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume313
Issue number4
DOIs
StatePublished - Oct 1 2017

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Liver Regeneration
Fatty Liver
Epidermal Growth Factor Receptor
Liver
Obese Mice
Survival
Transplantation
Wounds and Injuries
Hepatectomy
High Fat Diet
Gene Expression Profiling
Hydrodynamics
Mitosis
Transfection
Meta-Analysis
Adipose Tissue
Regeneration
Plasmids
Obesity
Mortality

Keywords

  • Epidermal growth factor receptor
  • Gene transfer
  • Hepatectomy
  • Microarray
  • Proliferation
  • Steatosis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Epidermal growth factor receptor restoration rescues the fatty liver regeneration in mice. / Zimmers, Teresa; Jin, Xiaoling; Zhang, Zongxiu; Jiang, Yanlin; Koniaris, Leonidas.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 313, No. 4, 01.10.2017, p. E440-E449.

Research output: Contribution to journalArticle

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