Epidermal G1‐chalone and transforming growth factor‐β are two different endogenous inhibitors of epidermal cell proliferation

K. Hartmut Richter, Ruben Schnapke, Matthias Clauss, Gerhard Fürstenberger, Detlef Hinz, Friedrich Marks

Research output: Contribution to journalArticle

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Abstract

Epidermal G1‐chalone and transforming growth factor‐β (TGFβ), two endogenous inhibitors of epidermal cell proliferation, were compared with regard to several effects on epidermis in vivo and in vitro. Both factors inhibited DNA labeling in a rat tongue epithelial cell line, with similar kinetics and half‐maximal effects at approximately 1 pg/ml (enriched chalone) and 1 ng/ml (TGFβ). For primary neonatal mouse keratinocytes, (TGFβ) was found to be a rather strong inhibitor of cell proliferation, whereas chalone showed only a weak effect on cells grown in medium containing 1.2 mM Ca2+ and no effect at all in the presence of 0.06 mM Ca2+. Vice versa, upon i.p. injection, only chalone was able to inhibit mouse epidermal DNA synthesis in vivo, whereas TGFβ had no effect at all. A moderate increase of transglutaminase activity in neonatal primary mouse keratinocytes was induced by both factors at concentrations of about 300 pg TGFβ/ml and 10 pg chalone fraction/ml. Chalone did not compete with [125]TGFβ for specific binding sites on primary murine keratinocytes. A polyclonal "chalone antiserum" did not interact with TGFβ, and a neutralizing TGFβ antibody that inhibited the effect of TGFβ on cell proliferation could not block the inhibitory effect of chalone on RTE2 cells. In contrast to TGFβ, epidermal G1‐chalone did not induce proliferation of NIH‐3T3 cells. These results indicate that epidermal G1‐chalone and TGFβ are two different inhibitors of epidermal cell proliferation.

Original languageEnglish (US)
Pages (from-to)496-504
Number of pages9
JournalJournal of cellular physiology
Volume142
Issue number3
DOIs
StatePublished - Mar 1990

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Cell proliferation
Transforming Growth Factors
Chalones
Cell Proliferation
Keratinocytes
G(1) chalone
Fibroblast Growth Factor 7
NIH 3T3 Cells
Transglutaminases
DNA
Tongue
Epidermis
Labeling
Rats
Immune Sera
Epithelial Cells
Binding Sites
Cells
Cell Line
Injections

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Epidermal G1‐chalone and transforming growth factor‐β are two different endogenous inhibitors of epidermal cell proliferation. / Richter, K. Hartmut; Schnapke, Ruben; Clauss, Matthias; Fürstenberger, Gerhard; Hinz, Detlef; Marks, Friedrich.

In: Journal of cellular physiology, Vol. 142, No. 3, 03.1990, p. 496-504.

Research output: Contribution to journalArticle

Richter, K. Hartmut ; Schnapke, Ruben ; Clauss, Matthias ; Fürstenberger, Gerhard ; Hinz, Detlef ; Marks, Friedrich. / Epidermal G1‐chalone and transforming growth factor‐β are two different endogenous inhibitors of epidermal cell proliferation. In: Journal of cellular physiology. 1990 ; Vol. 142, No. 3. pp. 496-504.
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