Epidermal platelet-activating factor receptor activation and ultraviolet B radiation result in synergistic tumor necrosis factor-alpha production

Jay E. Wolverton, Mohammed Al-Hassani, Yongxue Yao, Qiwei Zhang, Jeffrey B. Travers

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Ultraviolet B radiation (UVB) is a potent stimulator of epidermal cytokine production which has been implicated in photoaggravated dermatoses. In addition to cytokines such as tumor necrosis factor-α (TNF-α), UVB generates bioactive lipids including platelet-activating factor (PAF). Our previous studies have demonstrated that UVB-mediated production of keratinocyte TNF-α is in part due to PAF. The current studies use a human PAF-receptor (PAF-R) negative epithelial cell line transduced with PAF-Rs and PAF-R-deficient mice to demonstrate that activation of the epidermal PAF-R along with UVB irradiation results in a synergistic production of TNF-α. It should be noted that PAF-R effects are mimicked by the protein kinase C (PKC) agonist phorbol myristic acetate, and are inhibited by pharmacological antagonists of the PKC gamma isoenzyme. These studies suggest that concomitant PAF-R activation and UVB irradiation results in a synergistic production of the cytokine TNF-α which is mediated in part via PKC. These studies provide a novel potential mechanism for photosensitivity responses.

Original languageEnglish (US)
Pages (from-to)231-235
Number of pages5
JournalPhotochemistry and Photobiology
Volume86
Issue number1
DOIs
StatePublished - Jan 2010

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

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