Epigenetic regulation of alkaline phosphatase in human cells of the osteoblastic lineage

Jesus Delgado-Calle, Carolina Sañudo, Lydia Sánchez-Verde, Raúl J. García-Renedo, Jana Arozamena, José A. Riancho

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Epigenetic mechanisms play an important role in the tissue-specific regulation of gene expression. This study analyzed the relationship between tissue non-specific alkaline phosphatase (ALPL) gene expression and the methylation of a CpG island located in its proximal region. Gene expression was analyzed by real time RT-qPCR in primary human osteoblasts (hOBs), the osteoblastic cell line MG-63, the mammary cell line MCF-7, and bone tissue. DNA methylation was analyzed by qMSP in those cells and also in lining osteoblasts and in osteocytes obtained from human bone samples by laser-assisted capture. hOBs expressed much more ALPL mRNA than MG-63 cells (7.3 ± 3.2 vs. 0.2 ± 0.1 arbitrary units, respectively). hOBs showed a very weak DNA methylation (90%), whereas lining osteoblasts showed an intermediate degree of methylation (58 ± 13%). These results suggest an important role of DNA methylation in the regulation of ALPL expression through the osteoblast-osteocyte transition.

Original languageEnglish (US)
Pages (from-to)830-838
Number of pages9
JournalBone
Volume49
Issue number4
DOIs
StatePublished - Oct 2011
Externally publishedYes

Fingerprint

Cell Lineage
Osteoblasts
Epigenomics
Alkaline Phosphatase
DNA Methylation
Osteocytes
Methylation
Gene Expression
Bone and Bones
Cell Line
CpG Islands
Gene Expression Regulation
Breast
Lasers
Messenger RNA

Keywords

  • AzadC
  • DNA methylation
  • Epigenetics
  • Osteoblasts
  • Osteocytes

ASJC Scopus subject areas

  • Physiology
  • Endocrinology, Diabetes and Metabolism
  • Histology

Cite this

Delgado-Calle, J., Sañudo, C., Sánchez-Verde, L., García-Renedo, R. J., Arozamena, J., & Riancho, J. A. (2011). Epigenetic regulation of alkaline phosphatase in human cells of the osteoblastic lineage. Bone, 49(4), 830-838. https://doi.org/10.1016/j.bone.2011.06.006

Epigenetic regulation of alkaline phosphatase in human cells of the osteoblastic lineage. / Delgado-Calle, Jesus; Sañudo, Carolina; Sánchez-Verde, Lydia; García-Renedo, Raúl J.; Arozamena, Jana; Riancho, José A.

In: Bone, Vol. 49, No. 4, 10.2011, p. 830-838.

Research output: Contribution to journalArticle

Delgado-Calle, J, Sañudo, C, Sánchez-Verde, L, García-Renedo, RJ, Arozamena, J & Riancho, JA 2011, 'Epigenetic regulation of alkaline phosphatase in human cells of the osteoblastic lineage', Bone, vol. 49, no. 4, pp. 830-838. https://doi.org/10.1016/j.bone.2011.06.006
Delgado-Calle J, Sañudo C, Sánchez-Verde L, García-Renedo RJ, Arozamena J, Riancho JA. Epigenetic regulation of alkaline phosphatase in human cells of the osteoblastic lineage. Bone. 2011 Oct;49(4):830-838. https://doi.org/10.1016/j.bone.2011.06.006
Delgado-Calle, Jesus ; Sañudo, Carolina ; Sánchez-Verde, Lydia ; García-Renedo, Raúl J. ; Arozamena, Jana ; Riancho, José A. / Epigenetic regulation of alkaline phosphatase in human cells of the osteoblastic lineage. In: Bone. 2011 ; Vol. 49, No. 4. pp. 830-838.
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