Erk pathways negatively regulate matrix mineralization

Shin jiro Kono, Yasushi Oshima, Kazuto Hoshi, Lynda Bonewald, Hiromi Oda, Kozo Nakamura, Hiroshi Kawaguchi, Sakae Tanaka

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Skeletal mineralization is an important step regulating the mechanical properties of the calcified tissues, but molecular events underlying mineralization still remain elusive. We examined the role of extracellular signal-regulated kinase (Erk) pathways in matrix mineralization of osteogenic cells both in vitro and in vivo. Matrix mineralization by preosteocytic MLO-A5 cells and osteoblastic MC3T3-E1 cells was increased by either PD98059 Mek inhibitor treatment or adenovirus vector-mediated dominant negative Ras (RasDN) expression and was suppressed by Erk activation by platelet-derived growth factor (PDGF) treatment or constitutively active Mek1 (MekCA) expression. Administration of adenovirus vectors carrying RasDN gene onto the calvaria of 1-day-old mice increased the mineralization of the tissues, while that of the MekCA adenovirus suppressed it. These results suggest that the Erk pathway is a negative regulator of the matrix mineralization both in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)68-74
Number of pages7
JournalBone
Volume40
Issue number1
DOIs
StatePublished - Jan 2007
Externally publishedYes

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Extracellular Signal-Regulated MAP Kinases
Adenoviridae
Platelet-Derived Growth Factor
Skull
Genes
In Vitro Techniques

Keywords

  • Erk
  • Mek
  • Mineralization
  • Osteoblast
  • Osteocyte
  • Ras

ASJC Scopus subject areas

  • Physiology
  • Hematology

Cite this

Kono, S. J., Oshima, Y., Hoshi, K., Bonewald, L., Oda, H., Nakamura, K., ... Tanaka, S. (2007). Erk pathways negatively regulate matrix mineralization. Bone, 40(1), 68-74. https://doi.org/10.1016/j.bone.2006.07.024

Erk pathways negatively regulate matrix mineralization. / Kono, Shin jiro; Oshima, Yasushi; Hoshi, Kazuto; Bonewald, Lynda; Oda, Hiromi; Nakamura, Kozo; Kawaguchi, Hiroshi; Tanaka, Sakae.

In: Bone, Vol. 40, No. 1, 01.2007, p. 68-74.

Research output: Contribution to journalArticle

Kono, SJ, Oshima, Y, Hoshi, K, Bonewald, L, Oda, H, Nakamura, K, Kawaguchi, H & Tanaka, S 2007, 'Erk pathways negatively regulate matrix mineralization', Bone, vol. 40, no. 1, pp. 68-74. https://doi.org/10.1016/j.bone.2006.07.024
Kono SJ, Oshima Y, Hoshi K, Bonewald L, Oda H, Nakamura K et al. Erk pathways negatively regulate matrix mineralization. Bone. 2007 Jan;40(1):68-74. https://doi.org/10.1016/j.bone.2006.07.024
Kono, Shin jiro ; Oshima, Yasushi ; Hoshi, Kazuto ; Bonewald, Lynda ; Oda, Hiromi ; Nakamura, Kozo ; Kawaguchi, Hiroshi ; Tanaka, Sakae. / Erk pathways negatively regulate matrix mineralization. In: Bone. 2007 ; Vol. 40, No. 1. pp. 68-74.
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