Erythrocytes are the major intravascular storage sites of nitrite in human blood

André Dejam, Christian J. Hunter, Mildred M. Pelletier, Lewis L. Hsu, Roberto Machado, Sruti Shiva, Gordon G. Power, Malte Kelm, Mark T. Gladwin, Alan N. Schechter

Research output: Contribution to journalArticle

235 Citations (Scopus)

Abstract

Plasma levels of nitrite ions have been used as an index of nitric oxide synthase (NOS) activity in vivo. Recent data suggest that nitrite is a potential intravascular repository for nitric oxide (NO), bioactivated by a nitrite reductase activity of deoxyhemoglobin. The precise levels and compartmentalization of nitrite within blood and erythrocytes have not been determined. Nitrite levels in whole blood and erythrocytes were determined using reductive chemiluminescence in conjunction with a ferricyanide-based hemoglobin oxidation assay to prevent nitrite destruction. This method yields sensitive and linear measurements of whole blood nitrite over 24 hours at room temperature. Nitrite levels measured in plasma, erythrocytes, and whole blood from 15 healthy volunteers were 121 plus or minus 9, 288 plus or minus 47, and 176 plus or minus 17 nM, indicating a surprisingly high concentration of nitrite within erythrocytes. The majority of nitrite in erythrocytes is located in the cytosol unbound to proteins. In humans, we found a significant artery-to-vein gradient of nitrite in whole blood and erythrocytes. Shear stress and acetylcholine-mediated stimulation of endothelial NOS significantly increased venous nitrite levels. These studies suggest a dynamic intravascular NO metabolism in which endothelial NOS-derived NO is stabilized as nitrite, transported by erythrocytes, and consumed during arterial-to-venous transit.

Original languageEnglish (US)
Pages (from-to)734-739
Number of pages6
JournalBlood
Volume106
Issue number2
DOIs
StatePublished - Jul 15 2005
Externally publishedYes

Fingerprint

Nitrites
Blood
Erythrocytes
Nitric Oxide
Nitric Oxide Synthase Type III
Nitrite Reductases
Plasmas
Chemiluminescence
Luminescence
Metabolism
Nitric Oxide Synthase
Cytosol
Acetylcholine
Shear stress
Veins
Assays
Healthy Volunteers
Hemoglobins
Arteries
Ions

ASJC Scopus subject areas

  • Immunology
  • Biochemistry
  • Hematology
  • Cell Biology

Cite this

Dejam, A., Hunter, C. J., Pelletier, M. M., Hsu, L. L., Machado, R., Shiva, S., ... Schechter, A. N. (2005). Erythrocytes are the major intravascular storage sites of nitrite in human blood. Blood, 106(2), 734-739. https://doi.org/10.1182/blood-2005-02-0567

Erythrocytes are the major intravascular storage sites of nitrite in human blood. / Dejam, André; Hunter, Christian J.; Pelletier, Mildred M.; Hsu, Lewis L.; Machado, Roberto; Shiva, Sruti; Power, Gordon G.; Kelm, Malte; Gladwin, Mark T.; Schechter, Alan N.

In: Blood, Vol. 106, No. 2, 15.07.2005, p. 734-739.

Research output: Contribution to journalArticle

Dejam, A, Hunter, CJ, Pelletier, MM, Hsu, LL, Machado, R, Shiva, S, Power, GG, Kelm, M, Gladwin, MT & Schechter, AN 2005, 'Erythrocytes are the major intravascular storage sites of nitrite in human blood', Blood, vol. 106, no. 2, pp. 734-739. https://doi.org/10.1182/blood-2005-02-0567
Dejam, André ; Hunter, Christian J. ; Pelletier, Mildred M. ; Hsu, Lewis L. ; Machado, Roberto ; Shiva, Sruti ; Power, Gordon G. ; Kelm, Malte ; Gladwin, Mark T. ; Schechter, Alan N. / Erythrocytes are the major intravascular storage sites of nitrite in human blood. In: Blood. 2005 ; Vol. 106, No. 2. pp. 734-739.
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