Essential role for focal adhesion kinase in regulating stress hematopoiesis

Sasidhar Vemula, Baskar Ramdas, Philip Hanneman, Joseph Martin, Hilary E. Beggs, Reuben Kapur

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Focal adhesion kinase (FAK) is a nonreceptor tyrosine kinase that has been extensively studied in fibroblasts; however its function in hematopoiesis remains an enigma. FAK is thought to be expressed in myeloid and erythroid progenitors, and its expression is enhanced in response to cytokines such as granulocyte macrophage colony-stimulating factor. Furthermore, bone marrow cells cultured in granulocyte macrophage colony-stimulating factor show active migration and chemoattractant-induced polarization, which correlates with FAK induction. While loss of FAK in mice results in embryonic lethality, we have deleted FAK in the adult bone marrow. We show an essential role for FAK in regulating hemolytic, myelotoxic, as well as acute inflammatory stress responses in vivo. In vitro, loss of FAK in erythroid and myeloid progenitor's results in impaired cytokine induced growth and survival, as well as defects in the activation and expression of antiapoptotic proteins caspase 3 and Bcl-x L. Additionally, reduced migration and adhesion of myeloid cells on extracellular matrix proteins, as well as impaired activation of Rac GTPase is also observed in the absence of FAK. Our studies reveal an essential role for FAK in integrating growth/survival and adhesion based functions in myeloid and erythroid cells predominantly under conditions of stress.

Original languageEnglish
Pages (from-to)4103-4115
Number of pages13
JournalBlood
Volume116
Issue number20
DOIs
StatePublished - Nov 18 2010

Fingerprint

Focal Adhesion Protein-Tyrosine Kinases
Hematopoiesis
Myeloid Cells
Granulocyte-Macrophage Colony-Stimulating Factor
Bone
Adhesion
Cytokines
Chemical activation
Cells
Erythroid Cells
Extracellular Matrix Proteins
GTP Phosphohydrolases
Chemotactic Factors
Growth
Caspase 3
Bone Marrow Cells
Protein-Tyrosine Kinases
Fibroblasts
Bone Marrow
Polarization

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Vemula, S., Ramdas, B., Hanneman, P., Martin, J., Beggs, H. E., & Kapur, R. (2010). Essential role for focal adhesion kinase in regulating stress hematopoiesis. Blood, 116(20), 4103-4115. https://doi.org/10.1182/blood-2010-01-262790

Essential role for focal adhesion kinase in regulating stress hematopoiesis. / Vemula, Sasidhar; Ramdas, Baskar; Hanneman, Philip; Martin, Joseph; Beggs, Hilary E.; Kapur, Reuben.

In: Blood, Vol. 116, No. 20, 18.11.2010, p. 4103-4115.

Research output: Contribution to journalArticle

Vemula, S, Ramdas, B, Hanneman, P, Martin, J, Beggs, HE & Kapur, R 2010, 'Essential role for focal adhesion kinase in regulating stress hematopoiesis', Blood, vol. 116, no. 20, pp. 4103-4115. https://doi.org/10.1182/blood-2010-01-262790
Vemula, Sasidhar ; Ramdas, Baskar ; Hanneman, Philip ; Martin, Joseph ; Beggs, Hilary E. ; Kapur, Reuben. / Essential role for focal adhesion kinase in regulating stress hematopoiesis. In: Blood. 2010 ; Vol. 116, No. 20. pp. 4103-4115.
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