Establishment and phenotypic analysis of an Mstn knockout rat

Hao Gu, Yong Cao, Bin Qiu, Zhiqiang Zhou, Ran Deng, Zhuang Chen, Rongfeng Li, Xueling Li, Qiang Wei, Xianzhu Xia, Weidong Yong

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Myostatin (Mstn) is an inhibitor of myogenesis, regulating the number and size of skeletal myocytes. In addition to its myogenic regulatory function, Mstn plays important roles in the development of adipose tissues and in metabolism. In the present study, an Mstn knockout rat model was generated using the zinc finger nuclease (ZFN) technique in order to further investigate the function and mechanism of Mstn in metabolism. The knockout possesses a frame shift mutation resulting in an early termination codon and a truncated peptide of 109 amino acids rather than the full 376 amino acids. The absence of detectable mRNA confirmed successful knockout of Mstn. Relative to wild-type (WT) littermates, Knockout (KO) rats exhibited significantly greater body weight, body circumference, and muscle mass. However, no significant differences in grip force was observed, indicating that Mstn deletion results in greater muscle mass but not greater muscle fiber strength. Additionally, KO rats were found to possess less body fat relative to WT littermates, which is consistent with previous studies in mice and cattle. The aforementioned results indicate that Mstn knockout increases muscle mass while decreasing fat content, leading to observed increases in body weight and body circumference. The Mstn knockout rat model provides a novel means to study the role of Mstn in metabolism and Mstn-related muscle hypertrophy.

Original languageEnglish (US)
Pages (from-to)115-122
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume477
Issue number1
DOIs
StatePublished - Aug 12 2016

Keywords

  • Knockout rats
  • Mstn
  • Muscle development
  • TGF-β

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Gu, H., Cao, Y., Qiu, B., Zhou, Z., Deng, R., Chen, Z., Li, R., Li, X., Wei, Q., Xia, X., & Yong, W. (2016). Establishment and phenotypic analysis of an Mstn knockout rat. Biochemical and Biophysical Research Communications, 477(1), 115-122. https://doi.org/10.1016/j.bbrc.2016.06.030