Estradiol enhances thiazide-sensitive NaCI cotransporter density in the apical plasma membrane of the distal convoluted tubule in ovariectomized rats

Jill W. Verlander, Tuan  Tran, Li Zhang, Mark R. Kaplan, Steven C. Hebert

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Recent data suggest that sex hormones affect the thiazide-sensitive NaCl cotransporter (TSC) density or binding capacity (Chen, Z., D.A. Vaughn, and D.D. Fanestil. 1994. J. Am. Soc. Nephrol. 5:1112-1119). Thus, we determined the effect of ovariectomy (OVX) and estrogen replacement on the ultrastructural localization of TSC in rat kidney using immunocytochemistry. Kidneys of intact female (CON) and OVX rats fed ad libitum for 6 and 9 wk or pair-fed for 9 wk were processed for transmission electron microscopy. Immunogold localization of rat TSC (rTSC1) demonstrated intense label in the apical plasma membrane of CON distal convoluted tubule (DCT). In OVX DCT, rTSC1 label and apical plasma membrane microprojections were decreased. Western blots of renal membrane protein from pair-fed CON and OVX revealed bands at 129-135 kD, but the OVX signal was reduced. Morphometric analyses demonstrated that injecting 10 μg/kg body weight 17β-estradiol subcutaneously 4x/wk in OVX rats restored DCT apical microprojections and label density for rTSC1. Thus, in OVX rats (a) rTSC1 immunoreactive renal membrane protein is reduced; (b) apical plasma membrane complexity and immunogold label for rTSC1 in DCT is decreased; and (c) estradiol replacement restores DCT ultrastructure and rTSC1 label to normal. We conclude that estrogen enhances the density of rTSC1 in the DCT, and may alter renal Na transport by this mechanism.

Original languageEnglish (US)
Pages (from-to)1661-1669
Number of pages9
JournalJournal of Clinical Investigation
Volume101
Issue number8
StatePublished - Apr 15 1998
Externally publishedYes

Fingerprint

Estradiol
Cell Membrane
Kidney
Membrane Proteins
Estrogen Replacement Therapy
Gonadal Steroid Hormones
Ovariectomy
Transmission Electron Microscopy
Estrogens
Western Blotting
Immunohistochemistry
Body Weight
thiazide receptor

Keywords

  • Distal tubule thiazide diuretics
  • Estradiol
  • Immunohistochemistry
  • Kidney ultrastructure

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Estradiol enhances thiazide-sensitive NaCI cotransporter density in the apical plasma membrane of the distal convoluted tubule in ovariectomized rats. / Verlander, Jill W.; Tran, Tuan ; Zhang, Li; Kaplan, Mark R.; Hebert, Steven C.

In: Journal of Clinical Investigation, Vol. 101, No. 8, 15.04.1998, p. 1661-1669.

Research output: Contribution to journalArticle

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