Estradiol induces class I alcohol dehydrogenase activity and mRNA in kidney of female rats

Mona Qulali, Ruth Ann Ross, David Crabb

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Rat kidney contains alcohol dehydrogenase (ADH) activity which appears to be similar or identical to the class I ADH expressed in liver. Both tissues contain a 1.6-kb transcript which hybridizes with an ADH cDNA under stringent conditions. Kidney ADH activity is responsive to estradiol. The enzyme activity in the kidneys of shamoperated and ovariectomized animals was the same. Treatment of either group of animals by intramuscular injection of estradiol (1 mg/kg body wt/day) for 10 days induced ADH activity in kidney two- to threefold, whether the activity was expressed as U/g tissue, U/g protein, or U/mg DNA. Estradiol induced kidney ADH mRNA in both ovariectomized and sham-operated rats approximately twofold. Thus, induction of ADH mRNA accounts for the increase in ADH activity. In situ hybridization indicated that the ADH mRNA was present in the inner cortex and medulla of the kidney. Methylation patterns of the ADH gene were examined. The gene resides in a methylated region of chromatin without any of the typical features of a HpaII tiny fragment (HTF) island. Two MspI sites flanking the transcription start site are undermethylated in liver compared with kidney and spleen. This suggests that methylation of this gene may play a role in the tissue-specific expression of ADH.

Original languageEnglish
Pages (from-to)406-413
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume288
Issue number2
DOIs
StatePublished - Aug 1 1991

Fingerprint

Alcohol Dehydrogenase
Rats
Estradiol
Kidney
Messenger RNA
Methylation
Genes
Tissue
Liver
Animals
Kidney Medulla
Kidney Cortex
Transcription Initiation Site
Intramuscular Injections
Enzyme activity
Islands
Chromatin
In Situ Hybridization
Spleen
Complementary DNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Estradiol induces class I alcohol dehydrogenase activity and mRNA in kidney of female rats. / Qulali, Mona; Ross, Ruth Ann; Crabb, David.

In: Archives of Biochemistry and Biophysics, Vol. 288, No. 2, 01.08.1991, p. 406-413.

Research output: Contribution to journalArticle

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