Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells

Barry S. Komm, Christopher M. Terpening, David J. Benz, Kimberlie A. Graeme, Alfred Gallegos, Murray Korc, Geoffrey L. Greene, Bert W. O'Malley, Mark R. Haussler

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Abstract

High specific activity estradiol labeled with iodine-125 was used to detect approximately 200 saturable, high-affinity (dissociation constant ≅ 1.0 nM) nuclear binding sites in rat (ROS 17/2.8) and human (HOS TE85) clonal osteoblast-like osteosarcoma cells. Of the steroids tested, only testosterone exhibited significant cross-reactivity with estrogen binding. RNA blot analysis with a complementary DNA probe to the human estrogen receptor revealed putative receptor transcripts of 6 to 6.2 kilobases in both rat and human osteosarcoma cells. Type I procollagen and transforming growth factor-β messenger RNA levels were enhanced in cultured human osteoblast-like cells treated with 1 nM estradiol. Thus, estrogen can act directly on osteoblasts by a receptor-mediated mechanism and thereby modulate the extracellular matrix and other proteins involved in the maintenance of skeletal mineralization and remodeling.

Original languageEnglish (US)
Pages (from-to)81-84
Number of pages4
JournalScience
Volume241
Issue number4861
DOIs
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • General

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    Komm, B. S., Terpening, C. M., Benz, D. J., Graeme, K. A., Gallegos, A., Korc, M., Greene, G. L., O'Malley, B. W., & Haussler, M. R. (1988). Estrogen binding, receptor mRNA, and biologic response in osteoblast-like osteosarcoma cells. Science, 241(4861), 81-84. https://doi.org/10.1126/science.3164526