Estrogen depletion alters mineralization regulation mechanisms in an ovariectomized monkey animal model

E. P. Paschalis, S. Gamsjaeger, K. Condon, K. Klaushofer, David Burr

Research output: Contribution to journalArticle

Abstract

Ovariectomized animal models have been extensively used in osteoporosis research due to the resulting loss of bone mass. The purpose of the present study was to test the hypothesis that estrogen depletion alters mineralization regulation mechanisms in an ovariectomized monkey animal model. To achieve this we used Raman microspectroscopy to analyze humeri from monkeys that were either SHAM-operated or ovariectomized (N = 10 for each group). Measurements were made as a function of tissue age and cortical surface (periosteal, osteonal, endosteal) based on the presence of calcein fluorescent double labels. In the present work we focused on osteoid seams (defined as a surface with evident calcein labels, 1 μm distance away from the mineralizing front, and for which the Raman spectra showed the presence of organic matrix but not mineral), as well as the youngest mineralized tissue between the second fluorescent label and the mineralizing front, 1 μm inwards from the front with the phosphate mineral peak evident in the Raman spectra (TA1). The spectroscopically determined parameters of interest were the relative glycosaminoglycan (GAG) and pyridinoline (Pyd) contents in the osteoid, and the mineral content in TA1. At all three cortical surfaces, significant correlations were evident in the SHAM-operated animals between osteoid GAG (negative) and Pyd content, and mineral content, unlike the OVX animals. These results suggest that in addition to the well-established effects on turnover rates and bone mass, estrogen depletion alters the regulation of mineralization by GAGs and Pyd.

Original languageEnglish (US)
Pages (from-to)279-284
Number of pages6
JournalBone
Volume120
DOIs
StatePublished - Mar 1 2019

Fingerprint

Haplorhini
Minerals
Estrogens
Animal Models
Glycosaminoglycans
Bone Remodeling
Humerus
Osteoporosis
Phosphates
Bone and Bones
Research
pyridinoline
fluorexon
salicylhydroxamic acid

Keywords

  • Bone formation
  • Estrogen
  • Glycosaminoglycans
  • Osteoporosis
  • Pyridinoline
  • Raman spectroscopy

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Histology

Cite this

Estrogen depletion alters mineralization regulation mechanisms in an ovariectomized monkey animal model. / Paschalis, E. P.; Gamsjaeger, S.; Condon, K.; Klaushofer, K.; Burr, David.

In: Bone, Vol. 120, 01.03.2019, p. 279-284.

Research output: Contribution to journalArticle

Paschalis, EP, Gamsjaeger, S, Condon, K, Klaushofer, K & Burr, D 2019, 'Estrogen depletion alters mineralization regulation mechanisms in an ovariectomized monkey animal model', Bone, vol. 120, pp. 279-284. https://doi.org/10.1016/j.bone.2018.11.004
Paschalis, E. P. ; Gamsjaeger, S. ; Condon, K. ; Klaushofer, K. ; Burr, David. / Estrogen depletion alters mineralization regulation mechanisms in an ovariectomized monkey animal model. In: Bone. 2019 ; Vol. 120. pp. 279-284.
@article{e3f3250ce7134daab4a9d157908a6414,
title = "Estrogen depletion alters mineralization regulation mechanisms in an ovariectomized monkey animal model",
abstract = "Ovariectomized animal models have been extensively used in osteoporosis research due to the resulting loss of bone mass. The purpose of the present study was to test the hypothesis that estrogen depletion alters mineralization regulation mechanisms in an ovariectomized monkey animal model. To achieve this we used Raman microspectroscopy to analyze humeri from monkeys that were either SHAM-operated or ovariectomized (N = 10 for each group). Measurements were made as a function of tissue age and cortical surface (periosteal, osteonal, endosteal) based on the presence of calcein fluorescent double labels. In the present work we focused on osteoid seams (defined as a surface with evident calcein labels, 1 μm distance away from the mineralizing front, and for which the Raman spectra showed the presence of organic matrix but not mineral), as well as the youngest mineralized tissue between the second fluorescent label and the mineralizing front, 1 μm inwards from the front with the phosphate mineral peak evident in the Raman spectra (TA1). The spectroscopically determined parameters of interest were the relative glycosaminoglycan (GAG) and pyridinoline (Pyd) contents in the osteoid, and the mineral content in TA1. At all three cortical surfaces, significant correlations were evident in the SHAM-operated animals between osteoid GAG (negative) and Pyd content, and mineral content, unlike the OVX animals. These results suggest that in addition to the well-established effects on turnover rates and bone mass, estrogen depletion alters the regulation of mineralization by GAGs and Pyd.",
keywords = "Bone formation, Estrogen, Glycosaminoglycans, Osteoporosis, Pyridinoline, Raman spectroscopy",
author = "Paschalis, {E. P.} and S. Gamsjaeger and K. Condon and K. Klaushofer and David Burr",
year = "2019",
month = "3",
day = "1",
doi = "10.1016/j.bone.2018.11.004",
language = "English (US)",
volume = "120",
pages = "279--284",
journal = "Bone",
issn = "8756-3282",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Estrogen depletion alters mineralization regulation mechanisms in an ovariectomized monkey animal model

AU - Paschalis, E. P.

AU - Gamsjaeger, S.

AU - Condon, K.

AU - Klaushofer, K.

AU - Burr, David

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Ovariectomized animal models have been extensively used in osteoporosis research due to the resulting loss of bone mass. The purpose of the present study was to test the hypothesis that estrogen depletion alters mineralization regulation mechanisms in an ovariectomized monkey animal model. To achieve this we used Raman microspectroscopy to analyze humeri from monkeys that were either SHAM-operated or ovariectomized (N = 10 for each group). Measurements were made as a function of tissue age and cortical surface (periosteal, osteonal, endosteal) based on the presence of calcein fluorescent double labels. In the present work we focused on osteoid seams (defined as a surface with evident calcein labels, 1 μm distance away from the mineralizing front, and for which the Raman spectra showed the presence of organic matrix but not mineral), as well as the youngest mineralized tissue between the second fluorescent label and the mineralizing front, 1 μm inwards from the front with the phosphate mineral peak evident in the Raman spectra (TA1). The spectroscopically determined parameters of interest were the relative glycosaminoglycan (GAG) and pyridinoline (Pyd) contents in the osteoid, and the mineral content in TA1. At all three cortical surfaces, significant correlations were evident in the SHAM-operated animals between osteoid GAG (negative) and Pyd content, and mineral content, unlike the OVX animals. These results suggest that in addition to the well-established effects on turnover rates and bone mass, estrogen depletion alters the regulation of mineralization by GAGs and Pyd.

AB - Ovariectomized animal models have been extensively used in osteoporosis research due to the resulting loss of bone mass. The purpose of the present study was to test the hypothesis that estrogen depletion alters mineralization regulation mechanisms in an ovariectomized monkey animal model. To achieve this we used Raman microspectroscopy to analyze humeri from monkeys that were either SHAM-operated or ovariectomized (N = 10 for each group). Measurements were made as a function of tissue age and cortical surface (periosteal, osteonal, endosteal) based on the presence of calcein fluorescent double labels. In the present work we focused on osteoid seams (defined as a surface with evident calcein labels, 1 μm distance away from the mineralizing front, and for which the Raman spectra showed the presence of organic matrix but not mineral), as well as the youngest mineralized tissue between the second fluorescent label and the mineralizing front, 1 μm inwards from the front with the phosphate mineral peak evident in the Raman spectra (TA1). The spectroscopically determined parameters of interest were the relative glycosaminoglycan (GAG) and pyridinoline (Pyd) contents in the osteoid, and the mineral content in TA1. At all three cortical surfaces, significant correlations were evident in the SHAM-operated animals between osteoid GAG (negative) and Pyd content, and mineral content, unlike the OVX animals. These results suggest that in addition to the well-established effects on turnover rates and bone mass, estrogen depletion alters the regulation of mineralization by GAGs and Pyd.

KW - Bone formation

KW - Estrogen

KW - Glycosaminoglycans

KW - Osteoporosis

KW - Pyridinoline

KW - Raman spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=85056724688&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056724688&partnerID=8YFLogxK

U2 - 10.1016/j.bone.2018.11.004

DO - 10.1016/j.bone.2018.11.004

M3 - Article

VL - 120

SP - 279

EP - 284

JO - Bone

JF - Bone

SN - 8756-3282

ER -