Estrogen loss upregulates hematopoiesis in the mouse: A mediating role of IL-6

R. L. Jilka, G. Passeri, G. Girasole, S. Cooper, J. Abrams, Hal Broxmeyer, S. C. Manolagas

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

We have previously demonstrated that ovariectomy causes an increase in the number of colony-forming unit granulocyte/macrophage (CFU-GM) and an upregulation of osteoclastogenesis in mice, both of which are mediated by interleukin-6 (IL-6). IL-6 is involved in the development of several hematopoietic progenitors, including the burst-forming unit-erythroid (BFU-E) and multipotent CFUs (CFU-GEMM). Therefore, we performed studies to examine if other hematopoietic progenitors, besides CFU-GM and their progeny, are affected by estrogen loss. We found that ovariectomy caused an increase in the number of CFU-GEMM and BFU-E, as well as an increase of CFU-GM in marrow cells of the femur. Administration of 17β-estradiol or neutralizing antibody against IL-6 prevented the ovariectomy-induced increase in the number of these progenitors in the marrow. Ovariectomy also caused an increase in the number of circulating lymphocytes, neutrophils, and monocytes, which were suppressed by administration of 17β-estradiol or the neutralizing antibody against IL-6; however, the number of circulating platelets was unaffected by loss of ovarian function. These data establish that, in addition to upregulation of osteoclastogenesis, loss of estrogens in the mouse causes widespread effects on hematopoiesis, which are apparently mediated by IL-6.

Original languageEnglish (US)
Pages (from-to)500-506
Number of pages7
JournalExperimental Hematology
Volume23
Issue number6
StatePublished - 1995
Externally publishedYes

Fingerprint

Hematopoiesis
Ovariectomy
Interleukin-6
Estrogens
Up-Regulation
Granulocyte-Macrophage Progenitor Cells
Myeloid Progenitor Cells
Erythroid Precursor Cells
Neutralizing Antibodies
Osteogenesis
Estradiol
Bone Marrow
Lymphocyte Count
Platelet Count
Femur
Monocytes
Neutrophils

Keywords

  • Bone marrow
  • Estrogen
  • Hematopoietic progenitors
  • Interleukin-6
  • Leukocytes

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Jilka, R. L., Passeri, G., Girasole, G., Cooper, S., Abrams, J., Broxmeyer, H., & Manolagas, S. C. (1995). Estrogen loss upregulates hematopoiesis in the mouse: A mediating role of IL-6. Experimental Hematology, 23(6), 500-506.

Estrogen loss upregulates hematopoiesis in the mouse : A mediating role of IL-6. / Jilka, R. L.; Passeri, G.; Girasole, G.; Cooper, S.; Abrams, J.; Broxmeyer, Hal; Manolagas, S. C.

In: Experimental Hematology, Vol. 23, No. 6, 1995, p. 500-506.

Research output: Contribution to journalArticle

Jilka, RL, Passeri, G, Girasole, G, Cooper, S, Abrams, J, Broxmeyer, H & Manolagas, SC 1995, 'Estrogen loss upregulates hematopoiesis in the mouse: A mediating role of IL-6', Experimental Hematology, vol. 23, no. 6, pp. 500-506.
Jilka RL, Passeri G, Girasole G, Cooper S, Abrams J, Broxmeyer H et al. Estrogen loss upregulates hematopoiesis in the mouse: A mediating role of IL-6. Experimental Hematology. 1995;23(6):500-506.
Jilka, R. L. ; Passeri, G. ; Girasole, G. ; Cooper, S. ; Abrams, J. ; Broxmeyer, Hal ; Manolagas, S. C. / Estrogen loss upregulates hematopoiesis in the mouse : A mediating role of IL-6. In: Experimental Hematology. 1995 ; Vol. 23, No. 6. pp. 500-506.
@article{c720f9af6eba4cc2a243ec19bf8447a9,
title = "Estrogen loss upregulates hematopoiesis in the mouse: A mediating role of IL-6",
abstract = "We have previously demonstrated that ovariectomy causes an increase in the number of colony-forming unit granulocyte/macrophage (CFU-GM) and an upregulation of osteoclastogenesis in mice, both of which are mediated by interleukin-6 (IL-6). IL-6 is involved in the development of several hematopoietic progenitors, including the burst-forming unit-erythroid (BFU-E) and multipotent CFUs (CFU-GEMM). Therefore, we performed studies to examine if other hematopoietic progenitors, besides CFU-GM and their progeny, are affected by estrogen loss. We found that ovariectomy caused an increase in the number of CFU-GEMM and BFU-E, as well as an increase of CFU-GM in marrow cells of the femur. Administration of 17β-estradiol or neutralizing antibody against IL-6 prevented the ovariectomy-induced increase in the number of these progenitors in the marrow. Ovariectomy also caused an increase in the number of circulating lymphocytes, neutrophils, and monocytes, which were suppressed by administration of 17β-estradiol or the neutralizing antibody against IL-6; however, the number of circulating platelets was unaffected by loss of ovarian function. These data establish that, in addition to upregulation of osteoclastogenesis, loss of estrogens in the mouse causes widespread effects on hematopoiesis, which are apparently mediated by IL-6.",
keywords = "Bone marrow, Estrogen, Hematopoietic progenitors, Interleukin-6, Leukocytes",
author = "Jilka, {R. L.} and G. Passeri and G. Girasole and S. Cooper and J. Abrams and Hal Broxmeyer and Manolagas, {S. C.}",
year = "1995",
language = "English (US)",
volume = "23",
pages = "500--506",
journal = "Experimental Hematology",
issn = "0301-472X",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Estrogen loss upregulates hematopoiesis in the mouse

T2 - A mediating role of IL-6

AU - Jilka, R. L.

AU - Passeri, G.

AU - Girasole, G.

AU - Cooper, S.

AU - Abrams, J.

AU - Broxmeyer, Hal

AU - Manolagas, S. C.

PY - 1995

Y1 - 1995

N2 - We have previously demonstrated that ovariectomy causes an increase in the number of colony-forming unit granulocyte/macrophage (CFU-GM) and an upregulation of osteoclastogenesis in mice, both of which are mediated by interleukin-6 (IL-6). IL-6 is involved in the development of several hematopoietic progenitors, including the burst-forming unit-erythroid (BFU-E) and multipotent CFUs (CFU-GEMM). Therefore, we performed studies to examine if other hematopoietic progenitors, besides CFU-GM and their progeny, are affected by estrogen loss. We found that ovariectomy caused an increase in the number of CFU-GEMM and BFU-E, as well as an increase of CFU-GM in marrow cells of the femur. Administration of 17β-estradiol or neutralizing antibody against IL-6 prevented the ovariectomy-induced increase in the number of these progenitors in the marrow. Ovariectomy also caused an increase in the number of circulating lymphocytes, neutrophils, and monocytes, which were suppressed by administration of 17β-estradiol or the neutralizing antibody against IL-6; however, the number of circulating platelets was unaffected by loss of ovarian function. These data establish that, in addition to upregulation of osteoclastogenesis, loss of estrogens in the mouse causes widespread effects on hematopoiesis, which are apparently mediated by IL-6.

AB - We have previously demonstrated that ovariectomy causes an increase in the number of colony-forming unit granulocyte/macrophage (CFU-GM) and an upregulation of osteoclastogenesis in mice, both of which are mediated by interleukin-6 (IL-6). IL-6 is involved in the development of several hematopoietic progenitors, including the burst-forming unit-erythroid (BFU-E) and multipotent CFUs (CFU-GEMM). Therefore, we performed studies to examine if other hematopoietic progenitors, besides CFU-GM and their progeny, are affected by estrogen loss. We found that ovariectomy caused an increase in the number of CFU-GEMM and BFU-E, as well as an increase of CFU-GM in marrow cells of the femur. Administration of 17β-estradiol or neutralizing antibody against IL-6 prevented the ovariectomy-induced increase in the number of these progenitors in the marrow. Ovariectomy also caused an increase in the number of circulating lymphocytes, neutrophils, and monocytes, which were suppressed by administration of 17β-estradiol or the neutralizing antibody against IL-6; however, the number of circulating platelets was unaffected by loss of ovarian function. These data establish that, in addition to upregulation of osteoclastogenesis, loss of estrogens in the mouse causes widespread effects on hematopoiesis, which are apparently mediated by IL-6.

KW - Bone marrow

KW - Estrogen

KW - Hematopoietic progenitors

KW - Interleukin-6

KW - Leukocytes

UR - http://www.scopus.com/inward/record.url?scp=0029013885&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029013885&partnerID=8YFLogxK

M3 - Article

C2 - 7768305

AN - SCOPUS:0029013885

VL - 23

SP - 500

EP - 506

JO - Experimental Hematology

JF - Experimental Hematology

SN - 0301-472X

IS - 6

ER -