Estrogen modulates mesenchyme-epidermis interactions in the adult nipple

Hsing Jung Wu, Ji Won Oh, Dan Spandau, Sunil Tholpady, Jesus Diaz, Laura J. Schroeder, Carlos D. Offutt, Adam B. Glick, Maksim V. Plikus, Sachiko Koyama, John Foley

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Maintenance of specialized epidermis requires signals from the underlying mesenchyme; however, the specific pathways involved remain to be identified. By recombining cells from the ventral skin of the K14-PTHrP transgenic mice [which overexpress parathyroid hormone-related protein (PTHrP) in their developing epidermis and mammary glands] with those from wild type, we show that transgenic stroma is sufficient to reprogram wild-type keratinocytes into nipplelike epidermis. To identify candidate nipple-specific signaling factors, we compared gene expression signatures of sorted Pdgfrα-positive ventral K14-PTHrP and wild-type fibroblasts, identifying differentially expressed transcripts that are involved in WNT, HGF, TGFβ, IGF, BMP, FGF and estrogen signaling. Considering that some of the growth factor pathways are targets for estrogen regulation, we examined the upstream role of this hormone in maintaining the nipple. Ablation of estrogen signaling through ovariectomy produced nipples with abnormally thin epidermis, and we identified TGFβ as a negatively regulated target of estrogen signaling. Estrogen treatment represses Tgfβ1 at the transcript and protein levels in K14-PTHrP fibroblasts in vitro, while ovariectomy increases Tgfb1 levels in K14- PTHrP ventral skin. Moreover, ectopic delivery of Tgfβ1 protein into nipple connective tissue reduced epidermal proliferation. Taken together, these results show that specialized nipple epidermis is maintained by estrogen-induced repression of TGFβ signaling in the local fibroblasts.

Original languageEnglish (US)
Pages (from-to)1498-1509
Number of pages12
JournalDevelopment (Cambridge)
Volume144
Issue number8
DOIs
StatePublished - Apr 15 2017

Fingerprint

Nipples
Parathyroid Hormone-Related Protein
Mesoderm
Epidermis
Estrogens
Fibroblasts
Ovariectomy
Skin
Human Mammary Glands
Keratinocytes
Transcriptome
Connective Tissue
Transgenic Mice
Intercellular Signaling Peptides and Proteins
Proteins
Maintenance
Hormones

Keywords

  • Epidermis
  • Estrogen receptor α
  • Fibroblast
  • Nipple
  • TGFβ

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

Estrogen modulates mesenchyme-epidermis interactions in the adult nipple. / Wu, Hsing Jung; Oh, Ji Won; Spandau, Dan; Tholpady, Sunil; Diaz, Jesus; Schroeder, Laura J.; Offutt, Carlos D.; Glick, Adam B.; Plikus, Maksim V.; Koyama, Sachiko; Foley, John.

In: Development (Cambridge), Vol. 144, No. 8, 15.04.2017, p. 1498-1509.

Research output: Contribution to journalArticle

Wu, HJ, Oh, JW, Spandau, D, Tholpady, S, Diaz, J, Schroeder, LJ, Offutt, CD, Glick, AB, Plikus, MV, Koyama, S & Foley, J 2017, 'Estrogen modulates mesenchyme-epidermis interactions in the adult nipple', Development (Cambridge), vol. 144, no. 8, pp. 1498-1509. https://doi.org/10.1242/dev.141630
Wu, Hsing Jung ; Oh, Ji Won ; Spandau, Dan ; Tholpady, Sunil ; Diaz, Jesus ; Schroeder, Laura J. ; Offutt, Carlos D. ; Glick, Adam B. ; Plikus, Maksim V. ; Koyama, Sachiko ; Foley, John. / Estrogen modulates mesenchyme-epidermis interactions in the adult nipple. In: Development (Cambridge). 2017 ; Vol. 144, No. 8. pp. 1498-1509.
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