Ethanol and negative feedback regulation of mesolimbic dopamine release in rats

R. R. Kohl, J. S. Katner, E. Chernet, W. J. McBride

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The objectives of this study were to examine the relationship between somatodendritic and terminal field dopamine (DA) release following manipulation of DA D2 receptors in the ventral tegmental area (VTA), systemic administration of ethanol, and inhibition of DA uptake in the nucleus accumbens (ACE). Perfusion of 5, 25 and 100 μM quinpirole (a D2 agonist), or sulpiride (a D2 antagonist) through the microdialysis probe in the VTA produced dose-related decreases or increases, respectively, in the extracellular levels of DA in both the VTA and ACE of adult Wistar rats. The IP administration of 2-3 g/kg ethanol produced a sustained increase in the extracellular levels of DA (150-200% of baseline) in the ACE for at least 2 h after injection, whereas only a transient increase was observed in the VTA. Local perfusion of the ACE with 100 μM GBR12909, a DA uptake inhibitor, elevated the extracellular levels of DA in the ACE to approximately 400% of baseline, but decreased the extracellular levels of DA in the VTA to approximately 50% of baseline. Overall, the results suggest that (a) there is an association between somatodendritic and terminal field DA release when D2 cell body autoreceptors in the VTA are manipulated, (b) elevating synaptic levels of DA in the terminal field activates a long-loop negative feedback system to the VTA, and (c) different mechanisms may be mediating the actions of ethanol on DA neuronal activity and terminal DA release.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalPsychopharmacology
Volume139
Issue number1-2
DOIs
StatePublished - 1998

Fingerprint

Ventral Tegmental Area
Dopamine
Ethanol
Perfusion
Dopamine Uptake Inhibitors
Quinpirole
Autoreceptors
Sulpiride
Dopamine D2 Receptors
Microdialysis
Nucleus Accumbens
Wistar Rats
Injections

Keywords

  • Dopamine D autoreceptors
  • Dopamine release
  • GBR12909
  • Nucleus accumbens
  • Quinpirole
  • Somatodendritic dopamine release
  • Sulpiride
  • Ventral tegmental area

ASJC Scopus subject areas

  • Pharmacology

Cite this

Ethanol and negative feedback regulation of mesolimbic dopamine release in rats. / Kohl, R. R.; Katner, J. S.; Chernet, E.; McBride, W. J.

In: Psychopharmacology, Vol. 139, No. 1-2, 1998, p. 79-85.

Research output: Contribution to journalArticle

Kohl, R. R. ; Katner, J. S. ; Chernet, E. ; McBride, W. J. / Ethanol and negative feedback regulation of mesolimbic dopamine release in rats. In: Psychopharmacology. 1998 ; Vol. 139, No. 1-2. pp. 79-85.
@article{271219c5900747c4a727657092b96c55,
title = "Ethanol and negative feedback regulation of mesolimbic dopamine release in rats",
abstract = "The objectives of this study were to examine the relationship between somatodendritic and terminal field dopamine (DA) release following manipulation of DA D2 receptors in the ventral tegmental area (VTA), systemic administration of ethanol, and inhibition of DA uptake in the nucleus accumbens (ACE). Perfusion of 5, 25 and 100 μM quinpirole (a D2 agonist), or sulpiride (a D2 antagonist) through the microdialysis probe in the VTA produced dose-related decreases or increases, respectively, in the extracellular levels of DA in both the VTA and ACE of adult Wistar rats. The IP administration of 2-3 g/kg ethanol produced a sustained increase in the extracellular levels of DA (150-200{\%} of baseline) in the ACE for at least 2 h after injection, whereas only a transient increase was observed in the VTA. Local perfusion of the ACE with 100 μM GBR12909, a DA uptake inhibitor, elevated the extracellular levels of DA in the ACE to approximately 400{\%} of baseline, but decreased the extracellular levels of DA in the VTA to approximately 50{\%} of baseline. Overall, the results suggest that (a) there is an association between somatodendritic and terminal field DA release when D2 cell body autoreceptors in the VTA are manipulated, (b) elevating synaptic levels of DA in the terminal field activates a long-loop negative feedback system to the VTA, and (c) different mechanisms may be mediating the actions of ethanol on DA neuronal activity and terminal DA release.",
keywords = "Dopamine D autoreceptors, Dopamine release, GBR12909, Nucleus accumbens, Quinpirole, Somatodendritic dopamine release, Sulpiride, Ventral tegmental area",
author = "Kohl, {R. R.} and Katner, {J. S.} and E. Chernet and McBride, {W. J.}",
year = "1998",
doi = "10.1007/s002130050692",
language = "English",
volume = "139",
pages = "79--85",
journal = "Psychopharmacology",
issn = "0033-3158",
publisher = "Springer Verlag",
number = "1-2",

}

TY - JOUR

T1 - Ethanol and negative feedback regulation of mesolimbic dopamine release in rats

AU - Kohl, R. R.

AU - Katner, J. S.

AU - Chernet, E.

AU - McBride, W. J.

PY - 1998

Y1 - 1998

N2 - The objectives of this study were to examine the relationship between somatodendritic and terminal field dopamine (DA) release following manipulation of DA D2 receptors in the ventral tegmental area (VTA), systemic administration of ethanol, and inhibition of DA uptake in the nucleus accumbens (ACE). Perfusion of 5, 25 and 100 μM quinpirole (a D2 agonist), or sulpiride (a D2 antagonist) through the microdialysis probe in the VTA produced dose-related decreases or increases, respectively, in the extracellular levels of DA in both the VTA and ACE of adult Wistar rats. The IP administration of 2-3 g/kg ethanol produced a sustained increase in the extracellular levels of DA (150-200% of baseline) in the ACE for at least 2 h after injection, whereas only a transient increase was observed in the VTA. Local perfusion of the ACE with 100 μM GBR12909, a DA uptake inhibitor, elevated the extracellular levels of DA in the ACE to approximately 400% of baseline, but decreased the extracellular levels of DA in the VTA to approximately 50% of baseline. Overall, the results suggest that (a) there is an association between somatodendritic and terminal field DA release when D2 cell body autoreceptors in the VTA are manipulated, (b) elevating synaptic levels of DA in the terminal field activates a long-loop negative feedback system to the VTA, and (c) different mechanisms may be mediating the actions of ethanol on DA neuronal activity and terminal DA release.

AB - The objectives of this study were to examine the relationship between somatodendritic and terminal field dopamine (DA) release following manipulation of DA D2 receptors in the ventral tegmental area (VTA), systemic administration of ethanol, and inhibition of DA uptake in the nucleus accumbens (ACE). Perfusion of 5, 25 and 100 μM quinpirole (a D2 agonist), or sulpiride (a D2 antagonist) through the microdialysis probe in the VTA produced dose-related decreases or increases, respectively, in the extracellular levels of DA in both the VTA and ACE of adult Wistar rats. The IP administration of 2-3 g/kg ethanol produced a sustained increase in the extracellular levels of DA (150-200% of baseline) in the ACE for at least 2 h after injection, whereas only a transient increase was observed in the VTA. Local perfusion of the ACE with 100 μM GBR12909, a DA uptake inhibitor, elevated the extracellular levels of DA in the ACE to approximately 400% of baseline, but decreased the extracellular levels of DA in the VTA to approximately 50% of baseline. Overall, the results suggest that (a) there is an association between somatodendritic and terminal field DA release when D2 cell body autoreceptors in the VTA are manipulated, (b) elevating synaptic levels of DA in the terminal field activates a long-loop negative feedback system to the VTA, and (c) different mechanisms may be mediating the actions of ethanol on DA neuronal activity and terminal DA release.

KW - Dopamine D autoreceptors

KW - Dopamine release

KW - GBR12909

KW - Nucleus accumbens

KW - Quinpirole

KW - Somatodendritic dopamine release

KW - Sulpiride

KW - Ventral tegmental area

UR - http://www.scopus.com/inward/record.url?scp=0031686916&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031686916&partnerID=8YFLogxK

U2 - 10.1007/s002130050692

DO - 10.1007/s002130050692

M3 - Article

C2 - 9768545

AN - SCOPUS:0031686916

VL - 139

SP - 79

EP - 85

JO - Psychopharmacology

JF - Psychopharmacology

SN - 0033-3158

IS - 1-2

ER -